ArticleVeterinary and comparative oncology2021
Ki-67 assessment-agreeability between immunohistochemistry and flow cytometry in canine lymphoma.
Article in Veterinary and comparative oncology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 13 citations in OpenAlex.
- Diagnosis and Classification of Primary Nodal Lymphomas in Dogs: A Consensus of the Oncology-Pathology Working Group.Veterinary and comparative oncology · 2025Review
- Proliferation in Minimal Invasive Samples of Canine Lymphomas: Ki67 Index in Previously Stained Cytology and Paired Cell Blocks.Veterinary sciences · 2025Article
- Evaluation of salivary Ki-67 in health and periodontitis.BMC oral health · 2025Article
- Intraoperative Frozen Section Biopsy for the Auxiliary Diagnosis of Transmural Intestinal Intermediate T-Cell Canine Lymphoma.Veterinary sciences · 2025Article
- Exploratory screening for micro-RNA biomarkers in canine multicentric lymphoma.Frontiers in veterinary science · 2024Article
- Flow Cytometric Features of B- and T-Lmphocytes in Reactive Lymph Nodes Compared to Their Neoplastic Counterparts in Dogs.Veterinary sciences · 2023Article
- Article
- Multicolor flow cytometric assessment of Ki67 expression and its diagnostic value in mature B-cell neoplasms.Frontiers in oncology · 2023Article
- TubularInternational journal of molecular sciences · 2022Article
- Clinical outcome and Ki67 evaluation in dogs with nodal small cell B-cell lymphoma diagnosed by flow cytometry.Journal of veterinary internal medicine · 2022Article
- Ki-67 assessment-agreeability between immunohistochemistry and flow cytometry in canine lymphoma.Veterinary and comparative oncology · 2021Article
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
Recent literature suggests a combination of flow cytometric determination of Ki-67 and immunophenotype as a reliable tool to classify canine lymphomas. Immunohistochemistry (IHC) on histological samples is the gold standard technique assessing Ki-67 index. Agreement between IHC and FCM derived Ki-67 indices has never been investigated. The aim of this study was to investigate the agreement between IHC and FCM in the assessment of Ki-67 expression/index, in order to evaluate whether FCM may serve as a non-invasive alternative method for the estimation of proliferative activity in canine lymphoma. Dogs with previously untreated canine lymphoma undergoing diagnostic lymphadenectomy were prospectively enrolled. Ki-67 expression/index was assessed by FCM and IHC and expressed as percentage of positive cells. 39 dogs classified by histopathology matched the inclusion criteria. With both methods, Ki-67 expression/index was higher in intermediate/high-grade lymphomas. Spearman's coefficient of correlation was ρ = 0.57; (95% CI0.33-0.75) suggesting a moderate correlation. A Bland-Altman plot revealed a negative constant bias of -3.55 (95% CI: -10.52 to 3.42) with limits of agreement from -45.71 to 38.61. The study confirmed agreement albeit with wide confidence intervals between the values of Ki-67 expression/index assessed with FCM and IHC. Discrepancies were observed in a subset of cases. Possible explanation could be that Ki-67 index in IHC is determined in the most proliferative areas of the slide, which could introduce kind of sampling bias, whereas FCM evaluates many more cells in cell suspension. Further studies are warranted to investigate this phenomenon.
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