ArticleJournal of mass spectrometry : JMS2021
Amidation/non-amidation top-down analysis of endogenous neuropeptide Y in brain tissue by nano flow liquid chromatography orbitrap Fourier transform mass spectrometry.
Article in Journal of mass spectrometry : JMS, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 5 citations in OpenAlex.
- Understanding peptide hormones: from precursor proteins to bioactive molecules.Trends in biochemical sciences · 2025Review
- Weight Gain With Advancing Age Is Controlled by the Muscarinic Acetylcholine Receptor M4 in Male Mice.Endocrinology · 2025Article
- Quantitative Analysis of Neuropeptide Y (NPY) and C-Terminal Glycine-Extended NPY by Mass Spectrometry and Their Localization in the Developing and Sexual Adult Mouse Brains.ACS chemical neuroscience · 2025Article
- Amidation/non-amidation top-down analysis of endogenous neuropeptide Y in brain tissue by nano flow liquid chromatography orbitrap Fourier transform mass spectrometry.Journal of mass spectrometry : JMS · 2021Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neuropeptide Y (NPY) is a transmitter molecule in nerve system, and it was an over 4-kDa large peptide with the C-terminal end amidation. NPY is biosynthesized through many maturation processes from a large pre-pro-peptide with peptide-cleavages and amidation that is important to study the biosynthesis regulation. Previously, it was reported that cathepsin L participates in the production of NPY and that cathepsin L generates both of amidated and non-amidated NPYs. However, the non-amidated NPY (NPY-COOH) has not been reported in brain tissues until now. In this study, endogenous NPY-COOH in mouse brain tissue was detected and identified by using nano flow liquid chromatography (nanoLC) orbitrap Fourier transform mass spectrometry (FT-MS) after the effective purification and separation of NPY-COOH from NPY-amide and other peptides using two different gel-filtration chromatography. Amidated NPY was eluted earlier than non-amidated NPY-COOH in the C18 reversed phase nanoLC and the silica-based gel-filtration chromatogram with hydrophobic interaction. The amount of endogenous NPY-COOH was about 0.05% of the matured NPY-amide amount in adult mouse brain.
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