Evidence map›Paper›PMID 33759040›Full record

ArticleAMB Express2021

Synergistic effect of pulsed electric fields and temperature on the inactivation of microorganisms.

Zeyao Yan, Li Yin, Chunjing Hao, Kefu Liu, Jian Qiu

Abstract read
In one paragraph

Article in AMB Express, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Review
  2. Thermal preconditioning of targeted tissue enhances pulsed field ablation lesions.Journal of interventional cardiac electrophysiology : an international journal of arrhythmias and pacing · 2026
    Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Permeabilization ofFoods (Basel, Switzerland) · 2025
    Article
  8. Article
  9. Review
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Zeyao YanDepartment of Light Sources and Illuminating Engineering; Academy for Engineering & Technology, Fudan University, Shanghai, 200433, China.
Li YinDepartment of Light Sources and Illuminating Engineering; Academy for Engineering & Technology, Fudan University, Shanghai, 200433, China.
Chunjing HaoDepartment of Light Sources and Illuminating Engineering; Academy for Engineering & Technology, Fudan University, Shanghai, 200433, China.
Kefu LiuDepartment of Light Sources and Illuminating Engineering; Academy for Engineering & Technology, Fudan University, Shanghai, 200433, China. kfliu@fudan.edu.cn.
Jian QiuDepartment of Light Sources and Illuminating Engineering; Academy for Engineering & Technology, Fudan University, Shanghai, 200433, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Pulsed electric fields (PEF) as a new pasteurization technology played an important role in the process of inactivating microorganisms. At the same time, temperature could promote the process of electroporation, and achieve better inactivation effect. This article studied the inactivation effect of PEF on Saccharomyces cerevisiae, Escherichia coli, and Bacillus velezensis under different initial temperatures (room temperature-24 [Formula: see text], 30 [Formula: see text], 40 [Formula: see text], 50 [Formula: see text]). From the inactivation results, it found temperature could reduce the critical electric field intensity for microbial inactivation. After the irreversible electroporation of microorganisms occurred, the nucleic acid content and protein content in the suspension increased with the inactivation rate because the cell membrane integrity was destroyed. We had proved that the electric field and temperature could promote molecular transport through the finite element simulation. Under the same initial temperature and electrical parameters (electric field intensity, pulse width, pulse number), the lethal effect on different microorganisms was Saccharomyces cerevisiae > Escherichia coli > Bacillus velezensis.

Indexed as

Critical electric field intensityInitial temperatureNucleic acid contentProtein contentPulsed electric fields

Identifiers

PMID33759040
PMCPMC7988035

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.