ArticleCurrent microbiology2021
Evaluation of Legiolert™ for the Detection of Legionella pneumophila and Comparison with Spread-Plate Culture and qPCR Methods.
Article in Current microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed.
- A Hybrid Occupational Risk Assessment ofMicroorganisms · 2026Article
- Article
- Interlaboratory comparison of culture- and PCR-based methods forApplied and environmental microbiology · 2025Article
- Comparison between Legiolert and real time PCR in the detection of Legionella pneumophila from environmental water samples.PloS one · 2025Article
- Quantitative Microbial Risk Assessment Framework Incorporating Water Ages withEnvironmental science & technology · 2024Article
- Water Quality Trade-offs for Risk Management Interventions in a Green Building.Environmental science : water research & technology · 2024Article
- Four buildings and a flush: Lessons from degraded water quality and recommendations on building water management.Environmental science and ecotechnology · 2024Article
- Evaluation of a Most Probable Number Method for Detection and Quantification ofPathogens (Basel, Switzerland) · 2022Article
- VariableMicroorganisms · 2022Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Legionella pneumophila, the organism responsible for Legionnaires' disease, a potentially lethal pneumonia, is an opportunistic bacterium spread via inhalation of contaminated, aerosolized water. The detection and control of L. pneumophila is crucial to reduce the risk it poses to human health. L. pneumophila is generally detected and quantified by the plating method, ISO 11731:2017 and by qPCR. ISO 11731 is based on the filtration of the water sample through a membrane, which is placed on selective agar medium, and after colony growth, presumptive Legionella are then confirmed by subculturing, serology, or PCR. Quantitative Polymerase Chain Reaction (qPCR) is based on the amplification of a DNA sequence specific to L. pneumophila, usually within the mip gene. The objective of this study was to compare these methods to a new, liquid culture method based on the Most Probable Number (MPN) technique, Legiolert™/Quanti-Tray® with data obtained with ISO 11731 and a viability quantitative qPCR (v-qPCR), for quantification of L. pneumophila in potable and non-potable waters. Data showed that the Legiolert method revealed concentrations of L. pneumophila greater than ISO 11731 and generally similar results to those of v-qPCR. The Legiolert method was highly specific and easy to use, representing a significant advancement in the quantification of L. pneumophila from potable and non-potable waters.
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Registered trials
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