ArticleThe journal of physical chemistry. B2021
Temperature-Responsive Peptide-Nucleotide Coacervates.
Article in The journal of physical chemistry. B, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- Complexation of Polypeptides and Polypeptoids with Nucleic Acids: Does Chirality Matter for Salt Stability and Morphology?Biomolecules · 2026Article
- Elucidating the Reciprocal Interplay between Supramolecular Polymer Cytoskeletons and Fuel-Dependent Synthetic Cells.Journal of the American Chemical Society · 2026Article
- Article
- Dynamic and Catalytic Multiphase Coacervates.Biomacromolecules · 2026Article
- Anomalous Liquid-Liquid Phase Separation Dynamics in Polymerization-Driven Complex Coacervation.ACS macro letters · 2026Article
- MgChem & bio engineering · 2026Article
- Programmable Coacervates Based on Minimalist Sticker-Spacer Frameworks: Chemical Design, Functions, and Emerging Applications.ACS applied materials & interfaces · 2026Review
- DNA affects the phenotype of fuel-dependent coacervate droplets.Nature communications · 2026Article
- Effect of Polymer Structure on the Thermodynamics of Polyelectrolyte Complex Micelle Formation.Macromolecules · 2026Article
- Decoding nucleic acid contributions to phase separation and ordering in biomolecular condensates.Nucleic acids research · 2026Article
- Article
- Quantification of redox thermodynamics shifts within coacervates.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Differential stability and dynamics of DNA-based and RNA-based coacervates affect non-enzymatic RNA chemistry.Nature communications · 2025Article
- Smart coacervate microdroplets: biomimetic design, material innovations, and emerging applications in biomacromolecule delivery.Bioactive materials · 2025Review
- RNA-peptide interactions tune the ribozyme activity within coacervate microdroplet dispersions.Nature communications · 2025Article
- Dual-Stimulus Programmed Multiphase Separation and Organization in Coacervate Droplets.Angewandte Chemie (International ed. in English) · 2025Article
- Peptide Coacervates Can Protect Sequestered Oligonucleotides from Nucleases and Release Them for Transcription and Translation.Biomacromolecules · 2025Article
- Coacervate-Based Delivery Systems: Bridging Fundamentals and Applications.ACS applied materials & interfaces · 2025Review
- Light-Responsive Mononucleotide Coacervates.Chemistry (Weinheim an der Bergstrasse, Germany) · 2025Article
- Histidine-rich enantiomeric peptide coacervates enhance antigen sequestration and presentation to T cells.Chemical science · 2025Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Coacervates are a type of liquid-liquid phase separated (LLPS) droplets that can serve as models of membraneless organelles (MLOs) in living cells. Peptide-nucleotide coacervates have been widely used to mimic properties of ribonucleoprotein (RNP) granules, but the thermal stability and the role of base stacking is still poorly understood. Here, we report a systematic investigation of coacervates formed by five different nucleoside triphosphates (NTPs) with poly-l-lysine and poly-l-arginine as a function of temperature. All studied combinations exhibit an upper critical solution temperature (UCST), and a temperature-dependent critical salt concentration, originating from a significant nonelectrostatic contribution to the mixing free energy. Both the enthalpic and entropic parts of this nonelectrostatic interaction decrease in the order G/A/U/C/T, in accordance with nucleobase stacking free energies. Partitioning of two dyes proves that the local hydrophobicity inside the peptide-nucleotide coacervates is different for every nucleoside triphosphate. We derive a simple relation between the temperature and salt concentration at the critical point based on a mean-field model of phase separation. Finally, when different NTPs are mixed with one common oppositely charged peptide, hybrid coacervates were formed, characterized by a single intermediate UCST and critical salt concentration. NTPs with lower critical salt concentrations can remain condensed in mixed coacervates far beyond their original critical salt concentration. Our results show that NTP-based coacervates have a strong temperature sensitivity due to base stacking interactions and that mixing NTPs can significantly influence the stability of condensates and, by extension, their bioavailability.
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