Evidence map›Paper›PMID 33755919›Full record

ArticleGenes & genomics2021

Genome-wide identification of GMP genes in Rosaceae and functional characterization of FaGMP4 in strawberry (Fragaria × ananassa).

Yuanxiu Lin, Jiahao Zhang, Lintai Wu, Yunting Zhang, Qing Chen, Mengyao Li, Yong Zhang, Ya Luo, Yan Wang, Xiaorong Wang and 1 more

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Article in Genes & genomics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
6.6field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 12 citations in OpenAlex.

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  6. Gene expression profiling inFrontiers in plant science · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 1 institution in 1 country.

Yuanxiu Lin *College of Horticulture, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.ORCID http://orcid.org/0000-0002-6542-7836
Jiahao Zhang *College of Horticulture, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Lintai WuCollege of Horticulture, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Yunting ZhangCollege of Horticulture, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Qing ChenCollege of Horticulture, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Mengyao LiCollege of Horticulture, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Yong ZhangCollege of Horticulture, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Ya LuoCollege of Horticulture, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Yan WangCollege of Horticulture, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Xiaorong WangCollege of Horticulture, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Haoru TangCollege of Horticulture, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. htang@sicau.edu.cn.
Sichuan Agricultural University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundGDP-D-mannose pyrophosphorylase (GMP) is one of the key enzymes determining ascorbic acid (AsA) biosynthesis. However, little information about GMP genes is currently available for the Rosaceae species, especially in the AsA-riched cultivated octoploid strawberry (Fragaria × ananassa).

objectiveTo identify the all the GMP genes in Rosaceae, as well as the predominant homologues and the role of GMP genes in strawberry AsA accumulation.

methodsIn the present study, we performed genome-wide identification and comprehensive analysis of the duplicated GMP genes in strawberry and other Rosaceae species by bioinformatics methods, the expression of the GMP genes from cultivated strawberry (Fragaria × ananassa, FaGMP) was specifically analyzed by qPCR. Finally, the FaGMP4 was transiently overexpressed in strawberry to estimate the role of GMP in regulating AsA accumulation in strawberry.

resultsAs results, a total of 28 GMP genes were identified in the five Rosaceae species. The origins of duplication events analysis suggested that most GMP duplications in Rosaceae species were generated from whole genome duplication (WGD). The Ka/Ks ratio suggested that FaGMP genes underwent a stabilization selection. qPCR based expression analysis showed different patterns of FaGMP paralogs during fruit ripening, while FaGMP4 expressed higher in the variety containing higher AsA. Overexpression of FaGMP4 in strawberry significantly enhanced AsA accumulation. Furthermore, the expression of FaGMP4 under the treatment of blue and red light was largely increased in leaves while significantly inhibited in fruit. These results revealed the vital role of FaGMP4 in regulating AsA in strawberry.

Indexed as

PhylogenyAscorbic AcidFragariaFruitGene Expression Regulation, PlantGenome, PlantGenome-Wide Association StudyPlant LeavesRosaceaeAscorbic AcidAscorbic acidExpression analysisGDP-D-mannose pyrophosphorylaseStrawberry

Identifiers

PMID33755919
OpenAlexW3136747632

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.