ArticleJournal of experimental botany2021
Phosphorolytic degradation of leaf starch via plastidic α-glucan phosphorylase leads to optimized plant growth and water use efficiency over the diel phases of Crassulacean acid metabolism.
Article in Journal of experimental botany, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 17 citations in OpenAlex.
- A PROMOTER::LUCIFERASE reporter system reveals key elements of the circadian regulation of Crassulacean acid metabolism (CAM) in Kalanchoë laxiflora Baker.The Plant journal : for cell and molecular biology · 2026Article
- Clusia genomes shed light on the evolution and diversity of crassulacean acid metabolism physiotypes.Nature communications · 2026Article
- Synthetic crassulacean acid metabolism (SynCAM) for improving water-use efficiency in plants.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Review
- Regulation of Crassulacean acid metabolism at the protein level in Kalanchoë laxiflora.Plant physiology · 2025Article
- MdbZIP44-MdCPRF2-like-Horticulture research · 2024Article
- Article
- Elevated nocturnal respiratory rates in the mitochondria of CAM plants: current knowledge and unanswered questions.Annals of botany · 2023Article
- Integrative Proteomic and Phosphoproteomic Analyses Revealed the Regulatory Mechanism of the Response to Ultraviolet B Stress inACS omega · 2023Article
- Gene co-expression reveals the modularity and integration of C4 and CAM in Portulaca.Plant physiology · 2022Article
- Effects of chilling on the photosynthetic performance of the CAM orchidFrontiers in plant science · 2022Article
- Stomatal Responses to Light, COFrontiers in plant science · 2021Article
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
Abstract
In plants with Crassulacean acid metabolism (CAM), it has been proposed that the requirement for nocturnal provision of phosphoenolpyruvate as a substrate for CO2 uptake has resulted in a re-routing of chloroplastic starch degradation from the amylolytic route to the phosphorolytic route. To test this hypothesis, we generated and characterized four independent RNAi lines of the obligate CAM species Kalanchoë fedtschenkoi with a >10-fold reduction in transcript abundance of plastidic α-glucan phosphorylase (PHS1). The rPHS1 lines showed diminished nocturnal starch degradation, reduced dark CO2 uptake, a reduction in diel water use efficiency (WUE), and an overall reduction in growth. A re-routing of starch degradation via the hydrolytic/amylolytic pathway was indicated by hyperaccumulation of maltose in all rPHS1 lines. Further examination indicated that whilst operation of the core circadian clock was not compromised, plasticity in modulating net dark CO2 uptake in response to changing photoperiods was curtailed. The data show that phosphorolytic starch degradation is critical for efficient operation of the CAM cycle and for optimizing WUE. This finding has clear relevance for ongoing efforts to engineer CAM into non-CAM species as a means of boosting crop WUE for a warmer, drier future.
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