ArticleCommunications biology2021
High-resolution cryo-EM structure of photosystem II reveals damage from high-dose electron beams.
Article in Communications biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed.
- Effects of high light and elevated temperature on the structure of photosystem II isolated from Arabidopsis thaliana.Photosynthesis research · 2026Article
- An additional water is introduced into the manganese cluster during the formation of the SNature communications · 2026Article
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- Pulsed-electron illumination does not reduce beam damage for imaging biological macromolecules.Nature communications · 2026Article
- The structure of intact and active Photosystem II from Arabidopsis thaliana at 2.44 Å resolution.The New phytologist · 2026Article
- Structure and mechanism of the heterotetrameric ADP-glucose pyrophosphorylase essential for starch synthesis in plants.Science advances · 2026Article
- Amino acid and viral binding by the high-affinity Cationic Amino acid Transporter 1 (CAT1) from Mus musculus.Nature communications · 2026Article
- Article
- Structure, regulation and assembly of the photosynthetic electron transport chain.Nature reviews. Molecular cell biology · 2025Review
- Cryo-EM meets crystallography: A model-independent view of the heteronuclear MnProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Architecture and functional regulation of a plant PSII-LHCII megacomplex.Science advances · 2024Article
- Bicarbonate is a key regulator but not a substrate for OPhotosynthesis research · 2024Review
- Mutation-induced shift of the photosystem II active site reveals insight into conserved water channels.The Journal of biological chemistry · 2024Article
- Thermophilic cyanobacteria-exciting, yet challenging biotechnological chassis.Applied microbiology and biotechnology · 2024Review
- Article
- Effects of lipids on the rate-limiting steps in the dark-to-light transition of Photosystem II core complex ofFrontiers in plant science · 2024Article
- MDSPACE and MDTOMO Software for Extracting Continuous Conformational Landscapes from Datasets of Single Particle Images and Subtomograms Based on Molecular Dynamics Simulations: Latest Developments in ContinuousFlex Software Package.International journal of molecular sciences · 2023Article
- LipIDens: simulation assisted interpretation of lipid densities in cryo-EM structures of membrane proteins.Nature communications · 2023Article
- Evolutionary diversity of proton and water channels on the oxidizing side of photosystem II and their relevance to function.Photosynthesis research · 2023Review
- Water Networks in Photosystem II Using Crystalline Molecular Dynamics Simulations and Room-Temperature XFEL Serial Crystallography.Journal of the American Chemical Society · 2023Article
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7 authors.
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Abstract
Photosystem II (PSII) plays a key role in water-splitting and oxygen evolution. X-ray crystallography has revealed its atomic structure and some intermediate structures. However, these structures are in the crystalline state and its final state structure has not been solved. Here we analyzed the structure of PSII in solution at 1.95 Å resolution by single-particle cryo-electron microscopy (cryo-EM). The structure obtained is similar to the crystal structure, but a PsbY subunit was visible in the cryo-EM structure, indicating that it represents its physiological state more closely. Electron beam damage was observed at a high-dose in the regions that were easily affected by redox states, and reducing the beam dosage by reducing frames from 50 to 2 yielded a similar resolution but reduced the damage remarkably. This study will serve as a good indicator for determining damage-free cryo-EM structures of not only PSII but also all biological samples, especially redox-active metalloproteins.
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