ArticleScientific reports2021
Detection of differentially culturable tubercle bacteria in sputum using mycobacterial culture filtrates.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 38 citations in OpenAlex.
- Diagnostic value of phenotypic testing combined with molecular biology testing for tuberculosis.Scientific reports · 2026Article
- Prominence of Mycobacterium tuberculosis biomarkers among sputum culture-negative clinic attendees, independent of Ultra status.Journal of infection and public health · 2025Article
- Drug-induced differential culturability in diverse strains of Mycobacterium tuberculosis.Scientific reports · 2025Article
- Efficacy of Xpert MTB/RIF assay in detecting Mycobacterium tuberculosis in samples with different results by smear and culture in a coastal city with high incidence of tuberculosis.BMC infectious diseases · 2025Article
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- Yield of Facility-based Targeted Universal Testing for Tuberculosis With Xpert and Mycobacterial Culture in High-Risk Groups Attending Primary Care Facilities in South Africa.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2023Article
- Intranasal multivalent adenoviral-vectored vaccine protects against replicating and dormant M.tb in conventional and humanized mice.NPJ vaccines · 2023Article
- The performance of tongue swabs for detection of pulmonary tuberculosis.Frontiers in cellular and infection microbiology · 2023Article
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- Types and functions of heterogeneity in mycobacteria.Nature reviews. Microbiology · 2022Review
- Culture-Free Enumeration ofMicroorganisms · 2022Article
- Clinically encountered growth phenotypes of tuberculosis-causing bacilli and theirFrontiers in cellular and infection microbiology · 2022Review
- Efficacy of finafloxacin in a murine model of inhalational glanders.Frontiers in microbiology · 2022Article
- The detection of mixed tuberculosis infections using culture filtrate and resuscitation promoting factor deficient filtrate.Frontiers in cellular and infection microbiology · 2022Article
- Culture filtrate supplementation can be used to improveFrontiers in cellular and infection microbiology · 2022Article
- Drug resistant tuberculosis: Implications for transmission, diagnosis, and disease management.Frontiers in cellular and infection microbiology · 2022Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rapid detection of tuberculosis (TB) infection is paramount to curb further transmission. The gold standard for this remains mycobacterial culture, however emerging evidence confirms the presence of differentially culturable tubercle bacteria (DCTB) in clinical specimens. These bacteria do not grow under standard culture conditions and require the presence of culture filtrate (CF), from axenic cultures of Mycobacterium tuberculosis (Mtb), to emerge. It has been hypothesized that molecules such as resuscitation promoting factors (Rpfs), fatty acids and cyclic-AMP (cAMP) present in CF are responsible for the growth stimulatory activity. Herein, we tested the ability of CF from the non-pathogenic bacterium Mycobacterium smegmatis (Msm) to stimulate the growth of DCTB, as this organism provides a more tractable source of CF. We also interrogated the role of Mtb Rpfs in stimulation of DCTB by creating recombinant strains of Msm that express Mtb rpf genes in various combinations. CF derived from this panel of strains was tested on sputum from individuals with drug susceptible TB prior to treatment. CF from wild type Msm did not enable detection of DCTB in a manner akin to Mtb CF preparations and whilst the addition of RpfAB
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