ArticleScientific reports2021
Optical projection tomography as a quantitative tool for analysis of cell morphology and density in 3D hydrogels.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 19 citations in OpenAlex.
- Automated cell properties toolbox from 3D bioprinted hydrogel scaffolds via deep learning and optical coherence tomography.Biomedical optics express · 2025Article
- In-situ microscopy and digital image correlation to study the mechanical characteristics of polymer-based materials.Discover materials · 2025Review
- Aluminum-Free Borosilicate Glass Functionalized Hydrogels for Enhanced Dental Tissue Regeneration.Materials (Basel, Switzerland) · 2024Article
- Dynamic interface printing.Nature · 2024Article
- Exploring Label-Free Imaging Techniques with Copper Sulfide Microspheres for Observing Breast Cancer Cells.ACS omega · 2024Article
- Evaluation of Articular Cartilage Regeneration Properties of Decellularized Cartilage Powder/Modified Hyaluronic Acid Hydrogel Scaffolds.ACS omega · 2024Article
- An Experimental Platform for Tomographic Reconstruction of Tissue Images in Brightfield Microscopy.Sensors (Basel, Switzerland) · 2023Article
- Multifocal optical projection microscopy enables label-free 3D measurement of cardiomyocyte cluster contractility.Scientific reports · 2023Article
- Fabrication of mulberry leaf extract (MLE)- and tasar pupal oil (TPO)-loaded silk fibroin (SF) hydrogels and their antimicrobial properties.3 Biotech · 2023Article
- Murine cerebral organoids develop network of functional neurons and hippocampal brain region identity.iScience · 2021Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Assessing cell morphology and function, as well as biomaterial performance in cell cultures, is one of the key challenges in cell biology and tissue engineering (TE) research. In TE, there is an urgent need for methods to image actual three-dimensional (3D) cell cultures and access the living cells. This is difficult using established optical microscopy techniques such as wide-field or confocal microscopy. To address the problem, we have developed a new protocol using Optical Projection Tomography (OPT) to extract quantitative and qualitative measurements from hydrogel cell cultures. Using our tools, we demonstrated the method by analyzing cell response in three different hydrogel formulations in 3D with 1.5 mm diameter samples of: gellan gum (GG), gelatin functionalized gellan gum (gelatin-GG), and Geltrex. We investigated cell morphology, density, distribution, and viability in 3D living cells. Our results showed the usability of the method to quantify the cellular responses to biomaterial environment. We observed that an elongated morphology of cells, thus good material response, in gelatin-GG and Geltrex hydrogels compared with basic GG. Our results show that OPT has a sensitivity to assess in real 3D cultures the differences of cellular responses to the properties of biomaterials supporting the cells.
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