ArticleScientific reports2021
Selection for high levels of resistance to double-stranded RNA (dsRNA) in Colorado potato beetle (Leptinotarsa decemlineata Say) using non-transgenic foliar delivery.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 92 citations in OpenAlex.
- Advancing the adoption of RNA interference-based biopesticides.Nature plants · 2026Review
- RNAi: the next wave of green agriculture, challenges, and bridging the translational gap.Plant cell reports · 2026Review
- Chitosan-protected dsRNA increases efficacy against Helicoverpa armigera without compromising biosafety for pollinators.Pest management science · 2026Article
- RNA interference in crop protection: opportunities and challenges during the transition to commercialization.Pest management science · 2026Review
- Basic Research on Whitefly Molecular Biology: A Foundation for Innovative Pest Management Strategies.Current issues in molecular biology · 2026Review
- Screening Various Bacterial-Produced Double-Stranded RNAs for Managing Asian Soybean Rust Disease Caused byPlants (Basel, Switzerland) · 2026Article
- Norroa™ as a dsRNA biopesticide forFrontiers in insect science · 2026Review
- Next-generation insect pest management: genetic innovations and emerging biocontrol strategies.Frontiers in plant science · 2026Review
- Overview of polymeric polyplexes for dsRNA delivery in insects: complexation, stability, and design considerations.Frontiers in insect science · 2026Review
- Integrated Pest Management of Sclerotinia Stem Rot in Soybean: Current Strategies and Future Prospects.Journal of fungi (Basel, Switzerland) · 2025Review
- Bulk Segregant Analysis Reveals Genomic Regions Associated With Imidacloprid Resistance in the Colorado Potato Beetle.Ecology and evolution · 2025Article
- Control of Aedes albopictus populations by silencing of the vesicular GABA transporter (vgat) and the vesicular monoamine transporter (vmat) genes using recombinant Chlorella shRNA.Parasites & vectors · 2025Article
- Rootworm resistance to Bt associated with increased injury to corn pyramids combining Bt proteins and RNA interference.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- Environmental factors affecting RNAi efficacy: Temperature but not plant cultivar influences Colorado potato beetle's response to insecticidal dsRNA.Insect molecular biology · 2025Article
- Insecticide resistance management scenarios differ for RNA-based sprays and traits.Insect molecular biology · 2025Review
- Review
- Review
- Combined use of Chrysoperla rufilabris (Neuroptera: Chrysopidae) and Cry3Aa for improved control of Leptinotarsa decemlineata (Coleoptera: Chrysomelidae).Journal of insect science (Online) · 2025Article
- Designer circRNAPlant cell reports · 2025Article
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Authors and funding
18 authors at 11 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Insecticidal double-stranded RNAs (dsRNAs) silence expression of vital genes by activating the RNA interference (RNAi) mechanism in insect cells. Despite high commercial interest in insecticidal dsRNA, information on resistance to dsRNA is scarce, particularly for dsRNA products with non-transgenic delivery (ex. foliar/topical application) nearing regulatory review. We report the development of the CEAS 300 population of Colorado potato beetle (Leptinotarsa decemlineata Say) (Coleoptera: Chrysomelidae) with > 11,100-fold resistance to a dsRNA targeting the V-ATPase subunit A gene after nine episodes of selection using non-transgenic delivery by foliar coating. Resistance was associated with lack of target gene down-regulation in CEAS 300 larvae and cross-resistance to another dsRNA target (COPI β; Coatomer subunit beta). In contrast, CEAS 300 larvae showed very low (~ 4-fold) reduced susceptibility to the Cry3Aa insecticidal protein from Bacillus thuringiensis. Resistance to dsRNA in CEAS 300 is transmitted as an autosomal recessive trait and is polygenic. These data represent the first documented case of resistance in an insect pest with high pesticide resistance potential using dsRNA delivered through non-transgenic techniques. Information on the genetics of resistance and availability of dsRNA-resistant L. decemlineata guide the design of resistance management tools and allow research to identify resistance alleles and estimate resistance risks.
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