Evidence map›Paper›PMID 33753520›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2021

An enzyme-based biosensor for monitoring and engineering protein stability in vivo.

Chang Ren, Xin Wen, Jun Mencius, Shu Quan

Abstract read
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed.

  1. Enzyme-Integrated Hydrogels for Advanced Biological Applications.Polymer science & technology (Washington, D.C.) · 2026
    Review
  2. Review
  3. Review
  4. Article
  5. Article
  6. Article
  7. Review
  8. An intein-based biosensor to measure protein stability in vivo.Protein science : a publication of the Protein Society · 2024
    Article
  9. Article
  10. MEnTaT: A machine-learning approach for the identification of mutations to increase protein stability.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  11. Protein Stability: Enhancement and Measurement.Methods in molecular biology (Clifton, N.J.) · 2023
    Article
  12. Article
  13. Article
  14. Review
  15. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Chang RenState Key Laboratory of Bioreactor Engineering, Shanghai Collaborative Innovation Center for Biomanufacturing, East China University of Science and Technology, Shanghai 200237, China.ORCID 0000-0003-2449-4976
Xin WenState Key Laboratory of Bioreactor Engineering, Shanghai Collaborative Innovation Center for Biomanufacturing, East China University of Science and Technology, Shanghai 200237, China.
Jun MenciusState Key Laboratory of Bioreactor Engineering, Shanghai Collaborative Innovation Center for Biomanufacturing, East China University of Science and Technology, Shanghai 200237, China.
Shu QuanState Key Laboratory of Bioreactor Engineering, Shanghai Collaborative Innovation Center for Biomanufacturing, East China University of Science and Technology, Shanghai 200237, China shuquan@ecust.edu.cn.ORCID 0000-0002-6672-4947

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Protein stability affects the physiological functions of proteins and is also a desirable trait in many protein engineering tasks, yet improving protein stability is challenging because of limitations in methods for directly monitoring protein stability in cells. Here, we report an in vivo stability biosensor wherein a protein of interest (POI) is inserted into a microbial enzyme (CysG

Indexed as

Biosensing TechniquesProtein EngineeringProtein StabilityAcid Anhydride HydrolasesAcylphosphataseCatalytic DomainDirected Molecular EvolutionEscherichia coliFluorescenceHigh-Throughput Screening AssaysHumansMethyltransferasesMutationAcid Anhydride HydrolasesAcylphosphataseMethyltransferasesuroporphyrin-III C-methyltransferasebiosensordeep mutational scanningprotein engineeringprotein stability

Identifiers

PMID33753520
PMCPMC8020752

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.