ArticleProceedings of the National Academy of Sciences of the United States of America2021
The number of catalytic cycles in an enzyme's lifetime and why it matters to metabolic engineering.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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31 citing papers in PubMed.
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- Integrated metabolomic, proteomic, and transcriptomic analyses reveal the production of bioactive metabolites and antidiabetic effects in matureFrontiers in plant science · 2026Article
- Parameterization of cell-free systems with time-series data using KETCHUP.PLoS computational biology · 2025Article
- Thermostable Enzyme Variants in the Lower Mevalonate Pathway Improve Isoprenoid Production by Cell-Free Biocatalysis.ACS sustainable chemistry & engineering · 2025Article
- Harnessing mass spectrometry-based proteomics for continuous directed evolution.Synthetic biology (Oxford, England) · 2025Article
- Impact of bronchoalveolar lavage from influenza A virus diseased pigs on neutrophil functions and growth of co-infecting pathogenic bacteria.Frontiers in immunology · 2024Article
- A multi-organ maize metabolic model connects temperature stress with energy production and reducing power generation.iScience · 2023Article
- Tryptophan extends the life of cytochrome P450.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Semirational engineering ofApplied and environmental microbiology · 2023Article
- Adapting enzymes to improve their functionality in plants: why and how.Biochemical Society transactions · 2023Review
- Recruitment of a Middling Promiscuous Enzyme Drives Adaptive Metabolic Evolution in Escherichia coli.Molecular biology and evolution · 2023Article
- Seven plant capacities to adapt to abiotic stress.Journal of experimental botany · 2023Review
- Directed Evolution of Aerotolerance in Sulfide-Dependent Thiazole Synthases.ACS synthetic biology · 2023Article
- Respiratory energy demands and scope for demand expansion and destruction.Plant physiology · 2023Article
- Stability of enzyme immobilized on the nanofluidic channel surface.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2023Article
- Article
- Temperature-induced changes in Arabidopsis Rubisco activity and isoform expression.Journal of experimental botany · 2023Article
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10 authors.
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Abstract
Metabolic engineering uses enzymes as parts to build biosystems for specified tasks. Although a part's working life and failure modes are key engineering performance indicators, this is not yet so in metabolic engineering because it is not known how long enzymes remain functional in vivo or whether cumulative deterioration (wear-out), sudden random failure, or other causes drive replacement. Consequently, enzymes cannot be engineered to extend life and cut the high energy costs of replacement. Guided by catalyst engineering, we adopted catalytic cycles until replacement (CCR) as a metric for enzyme functional life span in vivo. CCR is the number of catalytic cycles that an enzyme mediates in vivo before failure or replacement, i.e., metabolic flux rate/protein turnover rate. We used estimated fluxes and measured protein turnover rates to calculate CCRs for ∼100-200 enzymes each from
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