ArticleBMC veterinary research2021
Freeze-thawing impairs the motility, plasma membrane integrity and mitochondria function of boar spermatozoa through generating excessive ROS.
Article in BMC veterinary research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 63 citations in OpenAlex.
- Cryopreservation of Human Spermatozoa Using Penetrating Cryoprotectant-Free Vitrification Medium Exhibits an Altered Proteomic Profile Compared to the Conventional Rapid Freezing Method.Reproductive sciences (Thousand Oaks, Calif.) · 2026Article
- Proteomic Profiling of Cryopreservation-Induced Alterations in Rhesus Macaque Sperm.Andrology · 2026Article
- Coenzyme Q10 supplementation enhances HSP70 gene expression and preserves mitochondrial function in cryopreserved Peranakan Ettawa goat spermatozoa.Veterinary world · 2026Article
- Long-term cryostorage does not impair human sperm motility: evidence from a three-decade cohort study.Journal of assisted reproduction and genetics · 2026Article
- Innovative nanotechnology approach using salicylic acid loaded chitosan nanoparticles to improve post-thaw sperm function, reduce apoptosis, and preserve ultrastructure in rams.Tropical animal health and production · 2026Article
- Effects of Sodium Butyrate on Sperm Function and Protein Acetylation in Fresh and Frozen-Thawed Boar Spermatozoa.Animals : an open access journal from MDPI · 2026Article
- Protective Effect of Intracellular Compounds of Lactobacillus plantarum on Goat Sperm Quality During Cryopreservation.Veterinary medicine and science · 2026Article
- Mitoregulin supports mitochondrial membrane integrity and protects against cardiac ischaemia-reperfusion injury.Cardiovascular research · 2026Article
- Cooling extender choice modulates the efficacy of freezing extenders for boar sperm cryopreservation.Animal reproduction · 2026Article
- Methyl Gallate Enhances Post-Thaw Boar Sperm Quality by Alleviating Oxidative Stress and Preserving Mitochondrial Function.Antioxidants (Basel, Switzerland) · 2025Article
- Upregulation of miR-151a-5p in high DFI sperm induces DNA damage and mitochondrial dysfunction by targeting INPP4B and VAMP1.Reproductive biology and endocrinology : RB&E · 2025Article
- Dose-Dependent Effects of L-Serine Supplementation on Boar Sperm Quality During Chilled and Cryopreserved Storage.Animals : an open access journal from MDPI · 2025Article
- Differential Metabolomic Signatures in Boar Sperm with Varying Liquid Preservation Capacities at 17 °C.Animals : an open access journal from MDPI · 2025Article
- Lipidomic Landscapes of Cryopreserved Sperm from Alpine and Spanish-Creole Bucks.Animals : an open access journal from MDPI · 2025Article
- Article
- Zearalenone Depresses Lactation Capacity Through the ROS-Mediated PI3K/AKT Pathway.Animals : an open access journal from MDPI · 2025Article
- Incubation of semen with human follicular fluid improves the antioxidant status and quality of spermatozoa after freezing-thawing.Reproduction & fertility · 2025Article
- Mesenchymal Stem Cell-Derived Small Extracellular Vesicle as A Novel Therapeutic Approach for Chemotherapy-Induced Male Infertility: A Review Article.International journal of fertility & sterility · 2025Article
- Thawing frozen foods: A comparative review of traditional and innovative methods.Comprehensive reviews in food science and food safety · 2025Review
- Asian palmyra palm (Open veterinary journal · 2025Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCryopreservation is an efficient way to store spermatozoa and is closely associated with the quality of sperm after the freeze-thaw process. During freeze-thaw cycling, excessive reactive oxygen species (ROS) are produced, and the effects of ROS on boar sperm during cryopreservation have not been identified.
resultsIn this study, we evaluated the quality of boar spermatozoa in different steps of cryopreservation (extension, cooling, and thawing for 30 min and 240 min) with or without boar-sperm antioxidant (N-acetylcysteine (NAC)). The ROS levels, sperm motility, plasma membrane integrity, mitochondrial activity, sperm chromatin structure, ATP content, and sperm apoptosis were assayed. After thawing, the ROS level and sperm apoptosis were significantly increased, and the sperm motility, plasma membrane integrity, mitochondrial activity, sperm chromatin structure, and ATP content were significantly impaired compared with those at the extension period and cooling period. Moreover, the addition of N-acetyl L-cysteine (NAC) reversed these changes.
conclusionThe freeze-thawing of boar spermatozoa impaired their motility, plasma membrane, mitochondrial activity, sperm chromatin structure and apoptosis by producing excessive ROS. Thus, the downregulation of ROS level by antioxidants, especially the NAC, is important for manufacturing frozen pig sperm to increase reproductive cells and livestock propagation, as well as to improve the application of frozen semen in pigs worldwide.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.