ArticleThe Plant cell2021
Seed-coat protective neolignans are produced by the dirigent protein AtDP1 and the laccase AtLAC5 in Arabidopsis.
Article in The Plant cell, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30 citing papers in PubMed, 55 citations in OpenAlex.
- Genome-wide identification, classification and expression analysis of laccase family members in Solanum lycopersicum.BMC plant biology · 2026Article
- Exploring the molecular landscape of environmental responses in the Antarctic plantFrontiers in plant science · 2026Article
- Oil body membrane proteins as sensors of salt stress in sunflower seedlings: a proteomic insight.Plant signaling & behavior · 2025Article
- Rainfall patterns during barley seed development underlie genomic variation for germination after flooding.Plant physiology · 2025Article
- A dirigent protein redirects extracellular terpenoid metabolism for defense against biotic challenges.Nature communications · 2025Article
- Physiological roles of lignins - tuning cell wall hygroscopy and biomechanics.The New phytologist · 2025Review
- Biochemical Analysis of Recombinant Pea Seed Coat-Specific Polyphenol Oxidase (Journal of agricultural and food chemistry · 2025Article
- Polymerization of proanthocyanidins under the catalysis of miR397a-regulated laccases in Salvia miltiorrhiza and Populus trichocarpa.Nature communications · 2025Article
- Multi-Omics Analysis Decodes Biosynthesis of Specialized Metabolites Constituting the Therapeutic Terrains ofInternational journal of molecular sciences · 2025Article
- Development of phytochemical genomics: From decoding metabolome to functional genomics and biotechnology of plant metabolism.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2025Review
- Genome-wide identification, classification, and expression profiling of LAC gene family in sesame.BMC plant biology · 2024Article
- Genome-Wide Identification and Characterization of the Laccase Gene Family inPlants (Basel, Switzerland) · 2024Article
- Expression of laccase and ascorbate oxidase affects lignin composition in Arabidopsis thaliana stems.Journal of plant research · 2024Article
- Extracellular vesicles of Norway spruce contain precursors and enzymes for lignin formation and salicylic acid.Plant physiology · 2024Article
- An intramolecular macrocyclase in plant ribosomal peptide biosynthesis.Nature chemical biology · 2024Article
- RNA-seq and metabolomic analyses of beneficial plant phenol biochemical pathways in red alder.Frontiers in plant science · 2024Article
- Two Arabidopsis promoters drive seed-coat specific gene expression in pennycress and camelina.Plant methods · 2023Article
- Multi-omic analyses reveal the unique properties of chia (Salvia hispanica) seed metabolism.Communications biology · 2023Article
- Pinoresinol rescues developmental phenotypes of Arabidopsis phenylpropanoid mutants overexpressingProceedings of the National Academy of Sciences of the United States of America · 2023Article
- ZmDRR206 Regulates Nutrient Accumulation in Endosperm through Its Role in Cell Wall Biogenesis during Maize Kernel Development.International journal of molecular sciences · 2023Article
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lignans/neolignans are generally synthesized from coniferyl alcohol (CA) in the cinnamate/monolignol pathway by oxidation to generate the corresponding radicals with subsequent stereoselective dimerization aided by dirigent proteins (DIRs). Genes encoding oxidases and DIRs for neolignan biosynthesis have not been identified previously. In Arabidopsis thaliana, the DIR AtDP1/AtDIR12 plays an essential role in the 8-O-4' coupling in neolignan biosynthesis by unequivocal structural determination of the compound missing in the atdp1 mutant as a sinapoylcholine (SC)-conjugated neolignan, erythro-3-{4-[2-hydroxy-2-(4-hydroxy-3-methoxyphenyl)-1-hydroxymethylethoxy]-3,5-dimethoxyphenyl}acryloylcholine. Phylogenetic analyses showed that AtDP1/AtDIR12 belongs to the DIR-a subfamily composed of DIRs for 8-8' coupling of monolignol radicals. AtDP1/AtDIR12 is specifically expressed in outer integument 1 cells in developing seeds. As a putative oxidase for neolignan biosynthesis, we focused on AtLAC5, a laccase gene coexpressed with AtDP1/AtDIR12. In lac5 mutants, the abundance of feruloylcholine (FC)-conjugated neolignans decreased to a level comparable to those in the atdp1 mutant. In addition, SC/FC-conjugated neolignans were missing in the seeds of mutants defective in SCT/SCPL19, an enzyme that synthesizes SC. These results strongly suggest that AtDP1/AtDIR12 and AtLAC5 are involved in neolignan biosynthesis via SC/FC. A tetrazolium penetration assay showed that seed coat permeability increased in atdp1 mutants, suggesting a protective role of neolignans in A. thaliana seeds.
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