ArticleThe Plant cell2021
DEAD-BOX RNA HELICASE 27 regulates microRNA biogenesis, zygote division, and stem cell homeostasis.
Article in The Plant cell, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Arabidopsis EMB2788/Npa1L1 is critical for pre-rRNA processing, ribosome biogenesis, and embryo development.The Plant journal : for cell and molecular biology · 2026Article
- Phylogenomic profile of exon-intron organization across angiosperms, their relationships with protein domains, and functional implications.Nature communications · 2026Article
- Characterization and Early Response of the DEAD Gene Family to Heat Stress in Tomato.Plants (Basel, Switzerland) · 2025Article
- The Use of Arabidopsis thaliana to Characterize the Production and Action Stages of the Plant MicroRNA Pathway.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Transcriptome-wide association mapping provides insights into the genetic basis and candidate genes governing flowering, maturity and seed weight in rice bean (Vigna umbellata).BMC plant biology · 2024Article
- miR-617 interacts with the promoter ofFrontiers in oncology · 2024Article
- microRNA biogenesis and stabilization in plants.Fundamental research · 2023Review
- Overexpression of AHL proteins enhances root hair production by altering the transcription of RHD6-downstream genes.Plant direct · 2023Article
- Hyponastic Leaves 1 Interacts with RNA Pol II to Ensure Proper Transcription of MicroRNA Genes.Plant & cell physiology · 2023Article
- A state-of-the-art review on the MicroRNAs roles in hematopoietic stem cell aging and longevity.Cell communication and signaling : CCS · 2023Review
- Functions and mechanisms of RNA helicases in plants.Journal of experimental botany · 2023Review
- Regulatory miPEP Open Reading Frames Contained in the Primary Transcripts of microRNAs.International journal of molecular sciences · 2023Review
- Recent Insights into Plant miRNA Biogenesis: Multiple Layers of miRNA Level Regulation.Plants (Basel, Switzerland) · 2023Review
- Identification of Pri-miRNA Stem-Loop Interacting Proteins in Plants Using a Modified Version of the Csy4 CRISPR Endonuclease.International journal of molecular sciences · 2022Article
- Biogenesis, conservation, and function of miRNA in liverworts.Journal of experimental botany · 2022Review
- Genome-wide identification, in silico characterization and expression analysis of the RNA helicase gene family in chickpea (C. arietinum L.).Scientific reports · 2022Article
- Mechanisms of MicroRNA Biogenesis and Stability Control in Plants.Frontiers in plant science · 2022Review
- Recent advances in the regulation of plant miRNA biogenesis.RNA biology · 2021Review
- Flowering plant embryos: How did we end up here?Plant reproduction · 2021Article
- DEAD-box helicases modulate dicing body formation inScience advances · 2021Article
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
After double fertilization, zygotic embryogenesis initiates a new life cycle, and stem cell homeostasis in the shoot apical meristem (SAM) and root apical meristem (RAM) allows plants to produce new tissues and organs continuously. Here, we report that mutations in DEAD-BOX RNA HELICASE 27 (RH27) affect zygote division and stem cell homeostasis in Arabidopsis (Arabidopsis thaliana). The strong mutant allele rh27-1 caused a zygote-lethal phenotype, while the weak mutant allele rh27-2 led to minor defects in embryogenesis and severely compromised stem cell homeostasis in the SAM and RAM. RH27 is expressed in embryos from the zygote stage, and in both the SAM and RAM, and RH27 is a nucleus-localized protein. The expression levels of genes related to stem cell homeostasis were elevated in rh27-2 plants, alongside down-regulation of their regulatory microRNAs (miRNAs). Further analyses of rh27-2 plants revealed reduced levels of a large subset of miRNAs and their pri-miRNAs in shoot apices and root tips. In addition, biochemical studies showed that RH27 associates with pri-miRNAs and interacts with miRNA-biogenesis components, including DAWDLE, HYPONASTIC LEAVES 1, and SERRATE. Therefore, we propose that RH27 is a component of the microprocessor complex and is critical for zygote division and stem cell homeostasis.
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