ArticleBMC infectious diseases2021
Development of a recombinase-aided amplification assay for rapid detection of human norovirus GII.4.
Article in BMC infectious diseases, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed, 23 citations in OpenAlex.
- A Rapid Detection Method for H3 Avian Influenza Viruses Based on RT-RAA.Animals : an open access journal from MDPI · 2024Article
- Current Trends in RNA Virus Detection via Nucleic Acid Isothermal Amplification-Based Platforms.Biosensors · 2024Review
- Development of a real-time recombinase-aided amplification assay for rapid and sensitive detection ofFrontiers in cellular and infection microbiology · 2024Article
- Molecular-electromechanical system for unamplified detection of trace analytes in biofluids.Nature protocols · 2023Review
- Rapid two-stage amplification in a single tube for simultaneous detection of norovirus GII and group a rotavirus.Journal of clinical laboratory analysis · 2023Article
- Rapid detection of mpox virus using recombinase aided amplification assay.Frontiers in cellular and infection microbiology · 2023Article
- Article
- Quick detection ofFrontiers in cellular and infection microbiology · 2022Article
- Rapid detection ofFrontiers in cellular and infection microbiology · 2022Article
- Development of a Lateral Flow Strip-Based Recombinase-Aided Amplification for ActiveFrontiers in microbiology · 2022Article
- A Recombinase Aided Amplification Assay for Rapid Detection of theFrontiers in cellular and infection microbiology · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundHuman noroviruses are one of the main causes of foodborne illnesses and represent a serious public health concern. Rapid and sensitive assays for human norovirus detection are undoubtedly necessary for clinical diagnosis, especially in regions without more sophisticated equipment.
methodThe rapid reverse transcription recombinase-aided amplification (RT-RAA) is a fast, robust and isothermal nucleic acid detection method based on enzyme reaction. This method can complete the sample detection at 39 °C in 30 min. In this study, we successfully established a rapid reverse transcription recombinase-aided amplification (RT-RAA) assay for the detection of human norovirus GII.4 and applied this assay to clinical samples, as well as comparison with commercial reverse transcription real-time fluorescence quantitative PCR (RT-qPCR).
resultsAt 95% probability, the detection sensitivity of RT-RAA was 3.425 log10 genomic copies (LGC)/reaction. Moreover, no cross-reaction was observed with other norovirus genogroups and other common foodborne viruses. Stool samples were examined by RT-RAA and reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR). Compared of RT-qPCR, kappa values for human norovirus detection with RT-RAA were 0.894 (p < 0.001), indicating that both assays were in agreement.
conclusionThis RT-RAA assay provides a rapid, specific, and sensitive assay for human norovirus detection and is suitable for clinical testing.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.