ArticleHeliyon2021
Comparison of multiplex cytokine assays in a pediatric cohort with epilepsy.
Article in Heliyon, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 2 of them syntheses that pooled it.
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Who cites it
13 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Sex-specific associations of depression with inflammatory markers: a systematic review and meta-analysis.Translational psychiatry · 2026Pooled it
- Proteomic profiling in personalized nutrition: a systematic review and methodological frameworks of randomized controlled trials.Frontiers in nutrition · 2026Pooled it
- Association of High-Dose Erythropoietin With Circulating Biomarkers and Neurodevelopmental Outcomes Among Neonates With Hypoxic Ischemic Encephalopathy: A Secondary Analysis of the HEAL Randomized Clinical Trial.JAMA network open · 2023Trial
- LUMINEX technology detects plasma inflammatory factor expression levels in children with severe and non-severe community acquired pneumonia.BMC pediatrics · 2026Article
- Mechanistically informed circulating biomarkers are associated with acquired epilepsy after neonatal brain injury.Journal of neuroinflammation · 2026Observational
- Effects of pre- and postnatal probiotic and ω-3 fatty acid supplementation on cytokine and chemokine responses to allergens and TLR ligands during infancy.Allergy, asthma, and clinical immunology : official journal of the Canadian Society of Allergy and Clinical Immunology · 2026Article
- Charting the Next Road Map for CSF Biomarkers in Alzheimer's Disease and Related Dementias.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2023Review
- Tear Fluid Biomarkers and Quality of Life in People with Type 2 Diabetes and Dry Eye Disease.Metabolites · 2023Article
- Systemic inflammation as a biomarker of seizure propensity and a target for treatment to reduce seizure propensity.Epilepsia open · 2023Review
- Cytokine responses to SARS-COV2 infection in mother-infant dyads: a systematic review and meta-analysis.Frontiers in pediatrics · 2023Review
- High-throughput proteomics: a methodological mini-review.Laboratory investigation; a journal of technical methods and pathology · 2022Review
- Risk modeling of femoral neck fracture based on geometric parameters of the proximal epiphysis.World journal of orthopedics · 2022Article
- Non-Invasive Transcutaneous Vagus Nerve Stimulation for the Treatment of Fibromyalgia Symptoms: A Study Protocol.Brain sciences · 2022Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
backgroundMultiplex analyses allow for detection of dozens of cytokines/chemokines in small sample volumes. Although several commercially available assay kits are available, there are no comparative data in plasma measurements among pediatric or epilepsy cohorts. NEW
methodCohort study of 38 children with epilepsy. We evaluated plasma levels of cytokines/chemokines using three different assays: Luminex® xMAP high-sensitivity (HS) and standard-sensitivity (SS) assays, and Meso-Scale Discovery (MSD). We calculated recovery rates of each analyte, correlation coefficients between assays, and level of agreement between measurements. We repeated analyses in a subset of samples after a single freeze-thaw cycle.
resultsAmong ten analytes common to all assays, HS had high recovery (<15% of values extrapolated or out-of- range [OOR]) for all analytes, SS for 50%, and MSD for 40%. While several analytes had a high correlation between assays, Bland-Altman plots demonstrated assays were not interchangeable. For most analytes, a single freeze-thaw cycle decreased cytokines/chemokine measurements. There was good correlation of measurements after a freeze-thaw cycle with acceptable agreement between measurements for six of 13 (46%) analytes using HS, one of 9 (11%) for SS, and none for MSD. COMPARISON WITH EXISTING
methodsHS assays may optimize yield in plasma for proteins of particular interest in epilepsy research, limit values extrapolated beyond the standard curve, and improve precision compared to other SS and MSD assays.
conclusionOur results demonstrate assay choice may be critical to study results and support the need for a standardized approach to biomarker assessment across epilepsy research and other domains.
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