ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2021
Droplet-Based Single-Cell Measurements of 16S rRNA Enable Integrated Bacteria Identification and Pheno-Molecular Antimicrobial Susceptibility Testing from Clinical Samples in 30 min.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 53 citations in OpenAlex.
- Harnessing microfluidics for microbiology: from bacteria-host interactions to emerging cancer therapies.Communications biology · 2026Review
- Pharmacists' Management of Urinary Tract Infection Symptoms in Community Pharmacy: Counseling Practices and Attitudes Toward Antibiotic Therapy.Pharmacy (Basel, Switzerland) · 2026Article
- Multiplexed, rapid phenotypic antibiotic susceptibility testing based on angle-resolved light scattering imaging of microfluidic droplets.Journal of advanced research · 2026Article
- Integrated iPRISM Direct-on-Urine Platform for Rapid UTI Diagnosis in a Double-Blind Clinical Trial.ACS measurement science au · 2026Article
- Rapid Bacteria Identification and Antimicrobial Susceptibility Testing via 5-Min Fluorescence In Situ Hybridization.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Rapid culture-free diagnosis of clinical pathogens via integrated microfluidic-Raman micro-spectroscopy.Nature communications · 2025Article
- Droplet-Based Microfluidics in Single-Bacterium Analysis: Advancements in Cultivation, Detection, and Application.Biosensors · 2025Review
- Advances in the isolation, cultivation, and identification of gut microbes.Military Medical Research · 2024Review
- Microfluidic technologies for advanced antimicrobial susceptibility testing.Biomicrofluidics · 2024Review
- Integrated Microfluidics for Single-Cell Separation and On-Chip Analysis: Novel Applications and Recent Advances.Small science · 2024Article
- Automated and miniaturized screening of antibiotic combinationsActa pharmaceutica Sinica. B · 2024Article
- Growth Independent Morphometric Machine Learning Workflow for Single-Cell Antimicrobial Susceptibility Testing ofFrontiers in imaging (Lausanne, Switzerland) · 2024Article
- Cellular immunity analysis by a modular acoustofluidic platform: CIAMAP.Science advances · 2023Article
- Recent Progress in Micro- and Nanotechnology-Enabled Sensors for Biomedical and Environmental Challenges.Sensors (Basel, Switzerland) · 2023Review
- Rapid Phenotypic Antimicrobial Susceptibility Testing Using a Coulter Counter and Proliferation Rate Discrepancy.ACS omega · 2023Article
- Advantage of precision metagenomics for urinary tract infection diagnostics.Frontiers in cellular and infection microbiology · 2023Article
- Single-cell pathogen diagnostics for combating antibiotic resistance.Nature reviews. Methods primers · 2023Article
- Rapid pathogen identification and phenotypic antimicrobial susceptibility directly from urine specimens.Scientific reports · 2022Article
- Rapid antibiotic susceptibility testing and species identification for mixed samples.Nature communications · 2022Article
- Combinatorial nanodroplet platform for screening antibiotic combinations.Lab on a chip · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Empiric broad-spectrum antimicrobial treatments of urinary tract infections (UTIs) have contributed to widespread antimicrobial resistance. Clinical adoption of evidence-based treatments necessitates rapid diagnostic methods for pathogen identification (ID) and antimicrobial susceptibility testing (AST) with minimal sample preparation. In response, a microfluidic droplet-based platform is developed for achieving both ID and AST from urine samples within 30 min. In this platform, fluorogenic hybridization probes are utilized to detect 16S rRNA from single bacterial cells encapsulated in picoliter droplets, enabling molecular identification of uropathogenic bacteria directly from urine in as little as 16 min. Moreover, in-droplet single-bacterial measurements of 16S rRNA provide a surrogate for AST, shortening the exposure time to 10 min for gentamicin and ciprofloxacin. A fully integrated device and screening workflow were developed to test urine specimens for one of seven unique diagnostic outcomes including the presence/absence of Gram-negative bacteria, molecular ID of the bacteriaas
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.