ReviewAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2021
Ice Inhibition for Cryopreservation: Materials, Strategies, and Challenges.
Review in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 100 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
100 citing papers in PubMed, 372 citations in OpenAlex.
- Cryopreservation technology for improving the stability of liposomes and its precise drug monitoring in clinical drug research.Drug delivery · 2026Article
- Development of Functional Synbiotic-Designed Sorbets as Carriers ofMolecules (Basel, Switzerland) · 2026Article
- Spontaneous Cyclic Levitation of Glycerol-Water Droplets at Low Pressures.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Low-toxicity vitrification of small-diameter arterial grafts by achieving a Cryo-Stasis state.Materials today. Bio · 2026Article
- Inhibition of Ice Recrystallization by Regulating the Surface Free Energy of Nanoparticles.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Molecular Mechanisms of Basil (Molecules (Basel, Switzerland) · 2026Article
- Cryopreservative Bioink Enables Direct Bioprinting of Adherent Cells.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Lessons From Drug Discovery for Cryoprotective Agent Design: An AI-Oriented Perspective.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- A Cryoprotectant-Compatible Nanoporous Platform for Stable and Scalable Delivery of Biopharmaceuticals.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Hyperactive ice-binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans.FEBS open bio · 2026Article
- A novel experimental platform to monitor solid/water interfaces under freeze-thaw cycles.Journal of microscopy · 2026Article
- Discovering naturally occurring antifreeze peptides from microbiome by integrating protein language models and molecular dynamics simulation.Journal of materials chemistry. B · 2026Article
- The quasi-liquid layer thickness controls clathrate hydrates' growth rate.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- The Effects of Graphene Oxide Nanoparticles on the Cryopreservation of Angora Buck Sperm.Molecules (Basel, Switzerland) · 2026Article
- Unconventional bioprinting modalities for advanced tissue biofabrication.Biomaterials · 2026Review
- Concurrent Formation of Ice Network within Mineral Colloids with Suppressed Volume Expansion.The journal of physical chemistry letters · 2026Article
- Dietary Theabrownins Improve Freeze-Thaw Quality of Grass Carp (Foods (Basel, Switzerland) · 2026Article
- Hydrogel Systems in Plant Germplasm Cryopreservation: A Comprehensive Review.Gels (Basel, Switzerland) · 2026Review
- Macroporous Alginate-PEG Hybrid Double Network Cryogels: Tuning Mechanics, Porosity, and Long-Term Growth Factor Release via Polymer Concentration, Ice Nucleation, and Sulfation.ACS applied bio materials · 2026Article
- Drying parameters and aging modulate protective properties of vitrified trehalose.bioRxiv : the preprint server for biology · 2026Article
40 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cryopreservation technology has developed into a fundamental and important supporting method for biomedical applications such as cell-based therapeutics, tissue engineering, assisted reproduction, and vaccine storage. The formation, growth, and recrystallization of ice crystals are the major limitations in cell/tissue/organ cryopreservation, and cause fatal cryoinjury to cryopreserved biological samples. Flourishing anti-icing materials and strategies can effectively regulate and suppress ice crystals, thus reducing ice damage and promoting cryopreservation efficiency. This review first describes the basic ice cryodamage mechanisms in the cryopreservation process. The recent development of chemical ice-inhibition molecules, including cryoprotectant, antifreeze protein, synthetic polymer, nanomaterial, and hydrogel, and their applications in cryopreservation are summarized. The advanced engineering strategies, including trehalose delivery, cell encapsulation, and bioinspired structure design for ice inhibition, are further discussed. Furthermore, external physical field technologies used for inhibiting ice crystals in both the cooling and thawing processes are systematically reviewed. Finally, the current challenges and future perspectives in the field of ice inhibition for high-efficiency cryopreservation are proposed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.