ArticleFrontiers in microbiology2021
Nile Red Incubation Time Before Reading Fluorescence Greatly Influences the Yeast Neutral Lipids Quantification.
Article in Frontiers in microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 18 citations in OpenAlex.
- Bipolar, not tetrapolar: mating system determination in Inonotus hispidus through genomic and phenotypic analysis.Applied microbiology and biotechnology · 2026Article
- Oleaginous yeasts from Brazilian Cerrado for sustainable biodiesel production using crude glycerol.Antonie van Leeuwenhoek · 2025Article
- A Quantitative Re-Assessment of Microencapsulation in (Pre-Treated) Yeast.Molecules (Basel, Switzerland) · 2024Article
- Extracts from Argentinian native plants reverse fluconazole resistance in Candida species by inhibiting the efflux transporters Mdr1 and Cdr1.BMC complementary medicine and therapies · 2022Article
- Key role of fluorescence quantum yield in Nile Red staining method for determining intracellular lipids in yeast strains.Biotechnology for biofuels and bioproducts · 2022Article
- Myricetin Disturbs the Cell Wall Integrity and Increases the Membrane Permeability ofJournal of microbiology and biotechnology · 2022Article
- Yeast Cells in Microencapsulation. General Features and Controlling Factors of the Encapsulation Process.Molecules (Basel, Switzerland) · 2021Review
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High-throughput screening methodologies to estimate lipid content in oleaginous yeasts use Nile red fluorescence in a given solvent and optimized excitation/emission wavelengths. However, Nile red fluorescence stabilization has been poorly analyzed, and high variability occurs when relative fluorescence is measured immediately or a few minutes after dye addition. The aim of this work was to analyze the fluorescence of Nile red at different incubation times using a variety of solvents and oleaginous/non-oleaginous yeast strains. We showed that fluorescence stabilization occurs between 20 and 30 min, depending on the strain and solvent. Therefore, we suggest that fluorescence measurements should be followed until stabilization, where Relative Fluorescence Units should be considered after stabilization for lipid content estimation.
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