ArticleAnalytical sciences : the international journal of the Japan Society for Analytical Chemistry2021
Zeptomole Detection of an Enzyme by a Simple Colorimetric Method.
Article in Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Advances in quantum dot-based fluorescent biosensors for enzyme detection: mechanistic insights and applications in disease.Mikrochimica acta · 2026Review
- Synergistic Catalysis of Gold-Platinum Alloy Nanozymes: A Novel Colorimetric Sensor for ALP Detection in Complex Biological Matrices.Pharmaceuticals (Basel, Switzerland) · 2025Article
- An overview of ELISA: a review and update on best laboratory practices for quantifying peptides and proteins in biological fluids.The Journal of international medical research · 2025Review
- Advanced Detection Method for Dengue NS1 Protein Using Ultrasensitive ELISA with Thio-NAD Cycling.Viruses · 2023Article
- Gastric Cancer Cell-Derived Exosomal GRP78 Enhances Angiogenesis upon Stimulation of Vascular Endothelial Cells.Current issues in molecular biology · 2022Article
- Article
- Modified ELISA for Ultrasensitive Diagnosis.Journal of clinical medicine · 2021Review
- Ultrasensitive Detection of SARS-CoV-2 Spike Proteins Using the Thio-NAD Cycling Reaction: A Preliminary Study before Clinical Trials.Microorganisms · 2021Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An enzyme immunoassay, in which an enzyme (e.g., alkaline phosphatase, ALP) is conjugated with an antibody, is a precise and simple protein detection method. Precise measurements of enzymes at low concentrations allow for ultrasensitive protein detection. The application of a phosphorylated substrate to ALP, followed by using a dephosphorylated substrate in thionicotinamide-adenine dinucleotide cycling, provides a simple and precise quantification of ALP. We describe a protocol for detecting ALP at the zeptomole level using a simple colorimetric method.
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Registered trials
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