Evidence map›Paper›PMID 33745222›Full record

ArticleJournal of clinical laboratory analysis2021

Altered expression of serum miR-106a, miR-19b, miR-17, and PTEN in patients with idiopathic membranous nephropathy.

Lina Wu, Xinpeng Zhang, Lin Luo, Xiaoying Li, Yong Liu, Xiaosong Qin

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of clinical laboratory analysis, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed, 1 pooled it
0.9field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 1 synthesis or guideline pooled it, 11 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Review
  4. Review
  5. Novel Biomarkers in Membranous Nephropathy.Frontiers in immunology · 2022
    Review
  6. Article
  7. Review
  8. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Lina WuDepartment of Laboratory Medicine, Shengjing Hospital of China Medical University, Shenyang, China.
Xinpeng ZhangDepartment of Laboratory Medicine, Shengjing Hospital of China Medical University, Shenyang, China.
Lin LuoDepartment of Laboratory Medicine, Shengjing Hospital of China Medical University, Shenyang, China.
Xiaoying LiDepartment of Laboratory Medicine, Shengjing Hospital of China Medical University, Shenyang, China.
Yong LiuDepartment of Laboratory Medicine, Shengjing Hospital of China Medical University, Shenyang, China.
Xiaosong QinDepartment of Laboratory Medicine, Shengjing Hospital of China Medical University, Shenyang, China.ORCID https://orcid.org/0000-0002-7171-6298
China Medical University · CNLiupanshui Normal University · CN

Funding

345 talent project" of Shengjing Hospital of China Medical UniversityLiaoning Province Central Government's special project to guide local scientific and technological development 2019JH6/10400009Liaoning Province Natural Science Foundation Project 20180550523Liaoning Province Natural Science Foundation Project 2019ZD0764Major Special Project of Construction Program of China Medical University in 2018 112/3110118034the National Science and Technology Major Project of China 2018ZX10302205
6 · The paper itself

Abstract

backgroundTo find new diagnostic markers for idiopathic membranous nephropathy (IMN) and also conduct preliminary explorations into the possible pathogenesis of IMN by comparing the expression of microRNA-451a (miR-451a), miR-106a, miR-19b, miR-17, and phosphatase and tensin homolog (PTEN) protein in the serum of patients with IMN and healthy controls.

methodsThe expression levels of miR-451a, miR-106a, miR-19b, and miR-17 in the serum of patients in the IMN group (n = 55, age: 50.2 ± 12.1 years) and the control group (n = 58, age 47.4 ± 13.1 years) were measured by quantitative real-time polymerase chain reaction (qRT-PCR), and the concentration of serum PTEN protein was determined by enzyme-linked immunosorbent assay (ELISA).

resultsCompared with the control group, the expression of miR-106a, miR-19b, and miR-17 was decreased significantly in the IMN group, whereas PTEN protein concentration was increased significantly in the IMN group. The areas under the receiver operating characteristic curve (AUC) of serum miR-106a, miR-19b, miR-17, and PTEN were 0.66 (95% confidence interval [CI], 0.56-0.76), 0.81 (95% CI, 0.73-0.89), 0.69 (95% CI, 0.59-0.79), and 0.86 (95% CI, 0.79-0.93), respectively. The level of serum PTEN protein was negatively correlated with the expression of miR-106a and miR-19b. PTEN concentration was positively correlated with serum urea (Urea), creatinine (Crea), cystatin C (Cysc), 24 h urine total protein (24 h-UP) and negatively correlated with albumin (Alb) and estimated glomerular filtration rate (eGFR).

conclusionsMiR-106a, miR-19b, miR-17, and PTEN are involved in the pathogenesis of IMN and may become new biomarkers for the diagnosis of IMN.

Indexed as

Gene Expression RegulationAlbuminsCase-Control StudiesCreatinineCystatin CFemaleGlomerular Filtration RateGlomerulonephritis, MembranousHumansMaleMicroRNAsMiddle AgedProteinuriaPTEN PhosphohydrolaseUreaAlbuminsCreatinineCystatin CMicroRNAsMIRN106 microRNA, humanMIRN17 microRNA, humanMIRN19A microRNA, humanPTEN PhosphohydrolasePTEN protein, humanUreaidiopathic membranous nephropathymicroRNA-106amicroRNA-17microRNA-19bmicroRNA-451aphosphatase and tensin homolog protein

Identifiers

PMID33745222
PMCPMC8059741
OpenAlexW3136797119

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.