Evidence map›Paper›PMID 33743780›Full record

ArticleActa veterinaria Scandinavica2021

Analysis of seroprevalence in target wildlife during the oral rabies vaccination programme in Lithuania.

Dainius Zienius, Janina Mickutė, Arnoldas Pautienius, Juozas Grigas, Arunas Stankevičius, Gediminas Pridotkas, Eugenijus Jacevičius, Jolita Kemeraitė, Ingrida Jacevičienė

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta veterinaria Scandinavica, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 50% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Dainius ZieniusLithuanian University of Health Sciences, Institute of Microbiology and Virology, Lithuania, Tilžės str. 18, 47181, Kaunas, Lithuania. dainius.zienius@lsmuni.lt.
Janina MickutėLithuanian University of Health Sciences, Institute of Microbiology and Virology, Lithuania, Tilžės str. 18, 47181, Kaunas, Lithuania.
Arnoldas PautieniusLithuanian University of Health Sciences, Institute of Microbiology and Virology, Lithuania, Tilžės str. 18, 47181, Kaunas, Lithuania.
Juozas GrigasLithuanian University of Health Sciences, Institute of Microbiology and Virology, Lithuania, Tilžės str. 18, 47181, Kaunas, Lithuania.
Arunas StankevičiusLithuanian University of Health Sciences, Institute of Microbiology and Virology, Lithuania, Tilžės str. 18, 47181, Kaunas, Lithuania.
Gediminas PridotkasNational Food and Veterinary Risk Assessment Institute, National Food and Veterinary Risk Assessment Institute, J. Kairiūkščio str. 10, 08409, Vilnius, Lithuania.
Eugenijus JacevičiusNational Food and Veterinary Risk Assessment Institute, National Food and Veterinary Risk Assessment Institute, J. Kairiūkščio str. 10, 08409, Vilnius, Lithuania.
Jolita KemeraitėNational Food and Veterinary Risk Assessment Institute, National Food and Veterinary Risk Assessment Institute, J. Kairiūkščio str. 10, 08409, Vilnius, Lithuania.
Ingrida JacevičienėNational Food and Veterinary Risk Assessment Institute, National Food and Veterinary Risk Assessment Institute, J. Kairiūkščio str. 10, 08409, Vilnius, Lithuania.
Lithuanian University of Health Sciences · LT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundRabies vaccination of wildlife carnivores is a powerful tool to prevent, control and eliminate rabies. The presence of neutralizing rabies antibodies in blood is considered a reliable indicator of adequate vaccination. The main purpose of the present study was to analyze the seroprevalence of specific antibodies in target populations of Lithuanian red fox (RF) and raccoon dog (RD) during the oral rabies vaccination (ORV) campaigns during the 2010-2019 period.

resultsOver the ten-year period, 7,261 RF and 2,146 RD sera samples were collected post-mortem in field conditions and tested using a commercial standardized enzyme-linked immunosorbent assay (ELISA) kit in Lithuania. In the ORV spring and autumn vaccination periods, 31.8% (20.3-43.4 95% CI - 95% confidence interval) and 31.7% (21.2-42.1 95% CI) of RF, and 34.1% (22.5-45.7 95% CI) and 34.7% (22.7-46.7 95% CI) of RD sera samples, respectively, were identified as ELISA-positive (seroconversion ≥ 0.5 EU/mL-Equivalent Units per Millilitre). The seroprevalence analysis in adult/ juvenile animal subpopulations indicated that 34.9% (27.2-42.5 95% CI) and 29.2% (20.3-37.9 95% CI) of RF, and 35.6% (25.2-46.0 95% CI) and 30.6% (20.2-40.9 95% CI) of RD sera samples, respectively, were identified as ELISA-positive (seroconversion ≥ 0.5 EU/mL). Statistically strong determinate correlations (r) between the serological results (pos.%) in RF adult/juvenile animal subpopulations (r = 0.937) and between RF and RD positive seroconvert (pos.%) sera samples during the spring vaccinations (r = 0.864) were demonstrated. In different ORV periods, 14-29% of RF and 7-25% of RD sera samples were identified as ELISA-negative (seroconversion < 0.5 EU/mL), but with low (0.125 < 0.49 EU/mL) antibody (Abs) titres.

conclusionsThe 2010-2019 ORV programme has been an effective tool in both RF and RD populations in Lithuania. The rabies-free status of Lithuania was self-declared in 2015 with only three rabies cases identified in buffer zones since then. The percentage of ELISA-positive serum samples (seroconversion ≥ 0.5 EU/mL) during the different periods of vaccination was similar in RF and RD populations-32% and 34% respectively. The identified seroconversion average of 21.5% in RF and 16% in RD sera samples were officially identified as ELISA-negative (seronversion < 0.5 EU/mL), but with low 0.125 < 0.49 EU/mL Abs titres. That low, but positive seroconversion participated in the formation of populations overall immune status and can influence the interpretation of oral vaccination efficacy.

Indexed as

Administration, OralAnimalsAnimals, WildAntibodies, ViralFoxesImmunization ProgramsLithuaniaRabiesRabies VaccinesRabies virusRaccoon DogsSeasonsSeroconversionSeroepidemiologic StudiesVaccinationAntibodies, ViralRabies VaccinesELISALithuaniaRabiesRaccoon dogRed foxSeroconversionVaccination

Identifiers

PMID33743780
PMCPMC7981835
OpenAlexW3137926154

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.