ArticleCell stress & chaperones2021
Heat stress promotes lipid accumulation by inhibiting the AMPK-PGC-1α signaling pathway in 3T3-L1 preadipocytes.
Article in Cell stress & chaperones, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 32 citations in OpenAlex.
- Heat stress in dairy buffalo: biometeorological, molecular, and adaptive strategies for climate change resilience in subtropical regions.Veterinary research communications · 2026Article
- Detection of genomic regions affecting thermotolerance traits in growing pigs during acute and chronic heat stress.Genetics, selection, evolution : GSE · 2025Article
- Neuromedin U Receptor NMUR3 Regulates Autophagy, Thereby Enhancing Thermal Tolerance inInternational journal of molecular sciences · 2025Article
- Chronic heat stress facilitates lipoprotein lipase expression and triglyceride biosynthesis in the adipose tissue of growing broiler chickens.Scientific reports · 2025Article
- Short-term heat exposure affects thermogenesis and mitophagy in goat brown adipocytes.BMC genomics · 2025Article
- Article
- RNA-seq reveals changes in the transcriptome of the breast muscle of adult female chickens in response to heat stress.BMC genomics · 2024Article
- AMPK-Mediated Hypolipidemic Effects of aNutrients · 2024Article
- Heat stress upregulates arachidonic acid to trigger autophagy in sertoli cells via dysfunctional mitochondrial respiratory chain function.Journal of translational medicine · 2024Article
- High water temperature raised the requirements of methionine for spotted seabass (Lateolabrax maculatus).Fish physiology and biochemistry · 2024Article
- Effect of temperature on growth, survival, and chronic stress responses of Arctic Grayling juveniles.Transactions of the American Fisheries Society · 2024Article
- Influence of different heat stress models on nutrient digestibility and markers of stress, inflammation, lipid, and protein metabolism in broilers.Poultry science · 2023Article
- Molecular Mechanism of m6A Methylation Modification GenesInternational journal of molecular sciences · 2023Article
- Temperature Fluctuations Modulate Molecular Mechanisms in Skeletal Muscle and Influence Growth Potential in Beef Steers.Journal of animal science · 2023Article
- Heat shock protein 27 regulates myogenic and self-renewal potential of bovine satellite cells under heat stress.Journal of animal science · 2023Article
- Dietary supplementation of dimethyl itaconate protects against chronic heat stress-induced growth performance impairment and lipid metabolism disorder in broiler chickens.Journal of animal science · 2023Article
- Article
- Feed Restriction Alleviates Chronic Thermal Stress-Induced Liver Oxidation and Damages via Reducing Lipid Accumulation in Channel Catfish (Antioxidants (Basel, Switzerland) · 2022Article
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8 authors at 1 institution in 1 country.
Funding
Abstract
Heat stress (HS) results in health problems in animals. This study was conducted to investigate the effect and the underlying mechanism of HS on the proliferation and differentiation process of 3T3-L1 preadipocytes. 3T3-L1 preadipocytes were treated at 37 °C or 41.5 °C. HS up-regulated the mRNA and protein expression level of heat shock protein 70 (HSP70). Furthermore, the proliferation of 3T3-L1 preadipocytes were significantly inhibited after HS treatment for 2 days. A large number of accumulated lipid droplets were observed under the microscope after HS treatment for 8 days. Notably, the result of oil red O staining showed that the number of lipid droplets increased significantly and the differentiation ability of the cells was enhanced after HS. Moreover, after 2 and 8 d of differentiation, HS increased the transcription levels of fat synthesis genes including peroxisome proliferators activated receptor γ (PPARγ), fatty acid binding protein 2 (AP2), fatty acid synthase (FAS) and CCAAT enhancer binding protein α (CEBPα) genes, while decreasing the transcription levels of lipid decomposition genes including ATGL and HSL genes. In addition, HS reduced the expression of AMPK and PGC-1α, as well as the dephosphorylation of AMPK. 5-Aminoimidazole-4-carboxamide ribonucleotide (AICAR) can eliminate HS induced lipogenesis by activating AMPK. These results indicated that HS inhibited the proliferation of 3T3-L1 preadipocytes and promoted lipid accumulation by inhibiting the AMPK-PGC-1α signaling pathway in 3T3-L1 preadipocytes. This work lays a theoretical foundation for improving the effect of HS on meat quality of livestock and provides a new direction for the prevention of obesity caused by HS.
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