Evidence map›Paper›PMID 33742394›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Preparation and Culture of Organotypic Hippocampal Slices for the Analysis of Brain Metastasis and Primary Brain Tumor Growth.

Ellina Schulz, Tim Hohmann, Urszula Hohmann, Ralf-Ingo Ernestus, Mario Löhr, Faramarz Dehghani, Carsten Hagemann

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Ellina SchulzDepartment of Neurosurgery, Tumorbiology Laboratory, University of Würzburg, Würzburg, Germany.
Tim HohmannInstitute of Anatomy and Cell Biology, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany.
Urszula HohmannInstitute of Anatomy and Cell Biology, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany.
Ralf-Ingo ErnestusDepartment of Neurosurgery, Tumorbiology Laboratory, University of Würzburg, Würzburg, Germany.
Mario LöhrDepartment of Neurosurgery, Tumorbiology Laboratory, University of Würzburg, Würzburg, Germany.
Faramarz Dehghani *Institute of Anatomy and Cell Biology, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany.
Carsten Hagemann *Department of Neurosurgery, Tumorbiology Laboratory, University of Würzburg, Würzburg, Germany. hagemann_c@ukw.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Brain metastasis is a major challenge for therapy and defines the end stage of tumor progression with a very limited patients' prognosis. Experimental setups that faithfully mimic these processes are necessary to understand the mechanism of brain metastasis and to develop new improved therapeutic strategies. Here, we describe an in vitro model, which closely resembles the in vivo situation. Organotypic hippocampal brain slice cultures (OHSCs) prepared from 3- to 8-day-old mice are well suited for neuro-oncology research including brain metastasis. The original morphology is preserved in OHSCs even after culture periods of several days to weeks. Tumor cells or cells of metastatic origin can be seeded onto OHSCs to evaluate micro-tumor formation, tumor cell invasion, or treatment response. We describe preparation and culture of OHSCs including the seeding of tumor cells. Finally, we show examples of how to treat the OHSCs for life-dead or immunohistochemical staining.

Indexed as

AnimalsBrain NeoplasmsGlioblastomaHippocampusImmunohistochemistryMiceNeoplasm MetastasisTissue Culture TechniquesBrain tumorCryosectionGlioblastomaGliomaImmunohistochemistryInvasionLife–dead stainingMetastasisOrganotypic hippocampal slice cultures (OHSCs)

Identifiers

PMID33742394

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.