Evidence map›Paper›PMID 33742256›Full record

ReviewPlant cell reports2022

State-of-the-Art in CRISPR Technology and Engineering Drought, Salinity, and Thermo-tolerant crop plants.

Kunchapu Chennakesavulu, Harshita Singh, Prabodh Kumar Trivedi, Mukesh Jain, Shri Ram Yadav

Abstract readReview
PubMed Publisher
In one paragraph

Review in Plant cell reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
3.2field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 55 citations in OpenAlex.

  1. Review
  2. CRISPR/Cas Genome Editing and Its Applications in Cereal Crop Improvement.Plant-environment interactions (Hoboken, N.J.) · 2026
    Review
  3. Dynamic water relations and alternative splicing in common bean under progressive soil drought and recovery.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026
    Article
  4. Review
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  14. Review
  15. Review
  16. Review
  17. Biotechnological Advances to Improve Abiotic Stress Tolerance in Crops.International journal of molecular sciences · 2022
    Review
  18. Review
  19. Article
  20. Improvement of heat stress tolerance in soybean (Frontiers in plant science · 2022
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Kunchapu ChennakesavuluDepartment of Biotechnology, Indian Institute of Technology, Roorkee, Uttarakhand, 247667, India.
Harshita SinghDepartment of Biotechnology, Indian Institute of Technology, Roorkee, Uttarakhand, 247667, India.
Prabodh Kumar TrivediCSIR-Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), Near Kukrail Picnic Spot, Lucknow, 226015, India.
Mukesh JainSchool of Computational and Integrative Sciences, Jawaharlal Nehru University, New Delhi, India.
Shri Ram YadavDepartment of Biotechnology, Indian Institute of Technology, Roorkee, Uttarakhand, 247667, India. shri.yadav@bt.iitr.ac.in.ORCID http://orcid.org/0000-0002-4083-7054
Indian Institute of Technology Roorkee · INCentral Institute of Medicinal and Aromatic Plants · INJawaharlal Nehru University · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

key messageOur review has described principles and functional importance of CRISPR-Cas9 with emphasis on the recent advancements, such as CRISPR-Cpf1, base editing (BE), prime editing (PE), epigenome editing, tissue-specific (CRISPR-TSKO), and inducible genome editing and their potential applications in generating stress-tolerant plants. Improved agricultural practices and enhanced food crop production using innovative crop breeding technology is essential for increasing access to nutritious foods across the planet. The crop plants play a pivotal role in energy and nutrient supply to humans. The abiotic stress factors, such as drought, heat, and salinity cause a substantial yield loss in crop plants and threaten food security. The most sustainable and eco-friendly way to overcome these challenges are the breeding of crop cultivars with improved tolerance against abiotic stress factors. The conventional plant breeding methods have been highly successful in developing abiotic stress-tolerant crop varieties, but usually cumbersome and time-consuming. Alternatively, the CRISPR/Cas genome editing has emerged as a revolutionary tool for making efficient and precise genetic manipulations in plant genomes. Here, we provide a comprehensive review of the CRISPR/Cas genome editing (GE) technology with an emphasis on recent advances in the plant genome editing, including base editing (BE), prime editing (PE), epigenome editing, tissue-specific (CRISPR-TSKO), and inducible genome editing (CRISPR-IGE), which can be used for obtaining cultivars with enhanced tolerance to various abiotic stress factors. We also describe tissue culture-free, DNA-free GE technology, and some of the CRISPR-based tools that can be modified for their use in crop plants.

Indexed as

CRISPR-Cas SystemsPlant BreedingDroughtsGene EditingGenome, PlantPlants, Genetically ModifiedSalinityTechnologyAbiotic stressBase editingCRISPR-IGECRISPR-TSKODNA-free GEEpigenome editingPrime editingTissue culture-free GE

Identifiers

PMID33742256
OpenAlexW3139302476

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.