ArticleScientific reports2021
Validation and verification of predictive salivary biomarkers for oral health.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 4 of them syntheses that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed, 4 syntheses or guidelines pooled it.
- Levels of IL-1β, MMP-8, and MMP-9 in the Saliva of Subjects With Periodontitis: A Systematic Review and Meta-Analysis.Journal of clinical laboratory analysis · 2025Pooled it
- Assessment of salivary matrix metalloproteinase (MMP8) and activated salivary matrix metalloproteinase (aMMP8) in periodontitis patients: a systematic review and meta-analysis.Frontiers in oral health · 2025Pooled it
- Evaluation of Salivary Biomarkers of Periodontal Disease Based on Smoking Status: A Systematic Review.International journal of environmental research and public health · 2022Pooled it
- Is TIMP-1 a biomarker for periodontal disease? A systematic review and meta-analysis.Journal of periodontal research · 2022Pooled it
- Diagnostic accuracy and smoking-driven modulation of salivary MMP-8, MMP-9, and HIF-1α in stage III grade B/C periodontitis: a cross-sectional study.Clinical oral investigations · 2026Article
- Machine learning-based analysis of oral rinse samples to identify candidate proteomic signatures for severe periodontitis: a pilot study.Scientific reports · 2026Article
- Non-invasive periodontal screening using self-reported-oral-health (SROH) questionnaire and salivary biomarkers: development and validation of machine learning models.BMC oral health · 2026Article
- TAM pathway proteins as novel salivary biomarkers for periodontitis.Journal of periodontology · 2026Observational
- Salivary and Serum Analytes and Their Associations with Self-rated Health Among Healthy Young Adults.International journal of behavioral medicine · 2026Article
- The Role of Salivary Diagnostics in Early Detection of Systemic and Oral Diseases: A Comprehensive Review.Cureus · 2025Review
- Saliva Versus All-Site Microbiome and Proteome Mapping in Periodontitis.Journal of clinical periodontology · 2025Article
- The influence of the menstrual cycle on inflammatory markers: the cytokines Il-1β, IL-6, and TNF-α in the gingival crevicular fluid.Journal of periodontal & implant science · 2025Article
- Evidence of Site-Specific Mucosal Autoantibody Secretion in Rheumatoid Arthritis.Arthritis & rheumatology (Hoboken, N.J.) · 2025Article
- Changes in MMP-9, T-SOD and SIRT-1 levels after non-surgical periodontal treatment.BMC oral health · 2025Article
- Meteorin-like levels of oral fluids in periodontal health and diseases: a comparative cross-sectional study.Clinical oral investigations · 2025Article
- Salivary and serum inflammatory biomarkers during periodontitis progression and after treatment.Journal of clinical periodontology · 2024Article
- Evaluating salivary MMP-8 as a biomarker for periodontal diseases: A systematic review and meta-analysis.Heliyon · 2024Article
- A REVIEW OF SALIVARY BIOMARKERS OF PERIODONTAL DISEASE.Annals of Ibadan postgraduate medicine · 2024Article
- A Roadmap for the Rational Use of Biomarkers in Oral Disease Screening.Biomolecules · 2024Review
- Oral Fluid Biomarkers for Peri-Implantitis: A Scoping Review.International dental journal · 2024Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Oral health is important not only due to the diseases emerging in the oral cavity but also due to the direct relation to systemic health. Thus, early and accurate characterization of the oral health status is of utmost importance. There are several salivary biomarkers as candidates for gingivitis and periodontitis, which are major oral health threats, affecting the gums. These need to be verified and validated for their potential use as differentiators of health, gingivitis and periodontitis status, before they are translated to chair-side for diagnostics and personalized monitoring. We aimed to measure 10 candidates using high sensitivity ELISAs in a well-controlled cohort of 127 individuals from three groups: periodontitis (60), gingivitis (31) and healthy (36). The statistical approaches included univariate statistical tests, receiver operating characteristic curves (ROC) with the corresponding Area Under the Curve (AUC) and Classification and Regression Tree (CART) analysis. The main outcomes were that the combination of multiple biomarker assays, rather than the use of single ones, can offer a predictive accuracy of > 90% for gingivitis versus health groups; and 100% for periodontitis versus health and periodontitis versus gingivitis groups. Furthermore, ratios of biomarkers MMP-8, MMP-9 and TIMP-1 were also proven to be powerful differentiating values compared to the single biomarkers.
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