ArticleNPJ biofilms and microbiomes2021
The biofilm matrix scaffold of Pseudomonas aeruginosa contains G-quadruplex extracellular DNA structures.
Article in NPJ biofilms and microbiomes, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 86 citations in OpenAlex.
- Imaging nucleic acids in biofilms.Essays in biochemistry · 2026Review
- Molecular insights into small RNA-mediated regulation of biofilm formation and multidrug resistance in Pseudomonas aeruginosa under zinc oxide nanoparticle exposure.Journal, genetic engineering & biotechnology · 2026Article
- Beyond probiotics: Lacticaseibacillus rhamnosus as a multi-target antagonist of Pseudomonas aeruginosa infections.Folia microbiologica · 2026Review
- Detecting extracellular G4 DNA motifs in biofilms through energy transfer between DNA-binding dyes TOTO™-1 and SYTO™60.Nucleic acids research · 2026Article
- Dual-action antimicrobial peptide therapy: disrupting biofilm formation and targeting bacterial DNA for enhanced treatment ofMicrobiology spectrum · 2026Article
- Probing biofilm development, stress response and heterogeneity-spectroscopic characterization of single and multi-species consortia.NPJ biofilms and microbiomes · 2026Article
- Cinnamic-Hydroxamic-Acid Derivatives Exhibit Antibiotic, Anti-Biofilm, and Supercoiling Relaxation Properties by Targeting Bacterial Nucleoid-Associated Protein HU.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Unravelling the noncanonical extracellular DNA structures in biofilm and NETosis.Nucleic acids research · 2026Review
- Stress-induced toxic genomic R-loops support biofilm extracellular matrix formation.Nature communications · 2025Article
- Beyond the matrix: rethinking antibiotic tolerance in CF biofilms using 3D models.NPJ biofilms and microbiomes · 2025Article
- Single-cell lysis patterns morphogenesis of eDNA in the matrix ofProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Hemin-binding DNA structures on the surface of bacteria promote extracellular electron transfer.Nucleic acids research · 2025Article
- Targeting Bacterial Biofilms on Medical Implants: Current and Emerging Approaches.Antibiotics (Basel, Switzerland) · 2025Review
- Dental biofilms contain DNase I-resistant Z-DNA and G-quadruplexes but alternative DNase overcomes this resistance.NPJ biofilms and microbiomes · 2025Article
- A DNP-Supported Solid-State NMR Approach to Study Nucleic Acids In Situ Reveals Berberine-Stabilized Hoogsteen Structures in Mitochondria.Angewandte Chemie (International ed. in English) · 2025Article
- Comparative analysis of biofilm structures in Salmonella Typhimurium DMC4 strain and its dam and seqA gene mutants using Fourier transform infrared spectroscopy (FT-IR) and Raman spectroscopy methods.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Neutrophil extracellular traps have active DNAzymes that promote bactericidal activity.Nucleic acids research · 2025Article
- Extracellular DNA-protein interactions.Current opinion in structural biology · 2024Review
- Biofilm matrix: a multifaceted layer of biomolecules and a defensive barrier against antimicrobials.Archives of microbiology · 2024Review
- Cyclic di-GMP as an antitoxin regulates bacterial genome stability and antibiotic persistence in biofilms.eLife · 2024Article
Corrections and comments
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Authors and funding
15 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Extracellular DNA, or eDNA, is recognised as a critical biofilm component; however, it is not understood how it forms networked matrix structures. Here, we isolate eDNA from static-culture Pseudomonas aeruginosa biofilms using ionic liquids to preserve its biophysical signatures of fluid viscoelasticity and the temperature dependency of DNA transitions. We describe a loss of eDNA network structure as resulting from a change in nucleic acid conformation, and propose that its ability to form viscoelastic structures is key to its role in building biofilm matrices. Solid-state analysis of isolated eDNA, as a proxy for eDNA structure in biofilms, reveals non-canonical Hoogsteen base pairs, triads or tetrads involving thymine or uracil, and guanine, suggesting that the eDNA forms G-quadruplex structures. These are less abundant in chromosomal DNA and disappear when eDNA undergoes conformation transition. We verify the occurrence of G-quadruplex structures in the extracellular matrix of intact static and flow-cell biofilms of P. aeruginosa, as displayed by the matrix to G-quadruplex-specific antibody binding, and validate the loss of G-quadruplex structures in vivo to occur coincident with the disappearance of eDNA fibres. Given their stability, understanding how extracellular G-quadruplex structures form will elucidate how P. aeruginosa eDNA builds viscoelastic networks, which are a foundational biofilm property.
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