ArticleNature communications2021
A computational workflow for the expansion of heterologous biosynthetic pathways to natural product derivatives.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Microbial Production of Alternative Proteins for Food Applications: Advances, Industrial Challenges, and Regulatory Status.Comprehensive reviews in food science and food safety · 2026Review
- De novo L-(+)-tartaric acid biosynthesis in multi-modular engineered yeasts.Nature communications · 2026Article
- Engineered microorganisms unlock a new era of diterpenoid biomanufacturing: recent advances and future perspectives.Bioresources and bioprocessing · 2026Review
- CBR-db: A Cheminformatic Database for Biochemical Reaction Analysis.ACS synthetic biology · 2026Article
- Genome-wide identification and characterization of methyltransferase involved in benzylisoquinoline alkaloids biosynthesis fromChinese herbal medicines · 2026Article
- Computational approaches to enzymatic reaction assignment: a review of methods, validations, and future directions.Briefings in bioinformatics · 2025Review
- Computational tools for nonnatural pathway design: Algorithms, applications, and challenges.Biodesign research · 2025Review
- Biosynfoni: a biosynthesis-informed and interpretable lightweight molecular fingerprint.Journal of cheminformatics · 2025Article
- Designing pathways for bioproducing complex chemicals by combining tools for pathway extraction and ranking.Nature communications · 2025Article
- Deletion of a polyketide synthaseFrontiers in microbiology · 2025Article
- High-throughput prediction of enzyme promiscuity based on substrate-product pairs.Briefings in bioinformatics · 2024Article
- Applications of synthetic biology in medical and pharmaceutical fields.Signal transduction and targeted therapy · 2023Review
- Pickaxe: a Python library for the prediction of novel metabolic reactions.BMC bioinformatics · 2023Article
- Biosynthesis of plant hemostatic dencichine in Escherichia coli.Nature communications · 2022Article
- Review
- Deep learning driven biosynthetic pathways navigation for natural products with BioNavi-NP.Nature communications · 2022Article
- Research Progress on the Synthetic Biology of Botanical Biopesticides.Bioengineering (Basel, Switzerland) · 2022Review
- Expanding biochemical knowledge and illuminating metabolic dark matter with ATLASx.Nature communications · 2022Article
- Complexity reduction and opportunities in the design, integration and intensification of biocatalytic processes for metabolite synthesis.Frontiers in bioengineering and biotechnology · 2022Review
- Catalytic promiscuity ofHorticulture research · 2022Article
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Authors and funding
5 authors.
Funding
Abstract
Plant natural products (PNPs) and their derivatives are important but underexplored sources of pharmaceutical molecules. To access this untapped potential, the reconstitution of heterologous PNP biosynthesis pathways in engineered microbes provides a valuable starting point to explore and produce novel PNP derivatives. Here, we introduce a computational workflow to systematically screen the biochemical vicinity of a biosynthetic pathway for pharmaceutical compounds that could be produced by derivatizing pathway intermediates. We apply our workflow to the biosynthetic pathway of noscapine, a benzylisoquinoline alkaloid (BIA) with a long history of medicinal use. Our workflow identifies pathways and enzyme candidates for the production of (S)-tetrahydropalmatine, a known analgesic and anxiolytic, and three additional derivatives. We then construct pathways for these compounds in yeast, resulting in platforms for de novo biosynthesis of BIA derivatives and demonstrating the value of cheminformatic tools to predict reactions, pathways, and enzymes in synthetic biology and metabolic engineering.
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