ArticleNature communications2021
Structural and dynamic mechanisms of CBF3-guided centromeric nucleosome formation.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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Who cites it
24 citing papers in PubMed, 36 citations in OpenAlex.
- Native yeast kinetochore structures identify an essential inner kinetochore interaction.Nature communications · 2026Article
- Native yeast kinetochore structures identify an essential inner kinetochore interaction.bioRxiv : the preprint server for biology · 2026Article
- Keeping it centered: decoding the activities that regulate yeast histone H3 variant Cse4 and confine it to centromeres.Frontiers in cell and developmental biology · 2026Review
- Artificial chromosome reorganization reveals high plasticity of the budding and fission yeast genomes.Genome biology · 2025Article
- Structural insights into the recognition of native nucleosomes by pioneer transcription factors.Current opinion in structural biology · 2025Review
- Force transmission through the inner kinetochore is enhanced by centromeric DNA sequences.eLife · 2025Article
- Concatemer-assisted stoichiometry analysis: targeted mass spectrometry for protein quantification.Life science alliance · 2025Article
- Beyond the mono-nucleosome.Biochemical Society transactions · 2025Review
- De novo assembly and single-molecule study of kinetochore-microtubule interactions.Frontiers in cell and developmental biology · 2025Article
- Structure of yeast RAVE bound to a partial VProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Structural insights into the cooperative nucleosome recognition and chromatin opening by FOXA1 and GATA4.Molecular cell · 2024Article
- Concatemer Assisted Stoichiometry Analysis (CASA): targeted mass spectrometry for protein quantification.bioRxiv : the preprint server for biology · 2024Article
- Structural mechanism of synergistic targeting of the CX3CR1 nucleosome by PU.1 and C/EBPα.Nature structural & molecular biology · 2024Article
- Humanization reveals pervasive incompatibility of yeast and human kinetochore components.G3 (Bethesda, Md.) · 2023Article
- Recognition of centromere-specific histone Cse4 by the inner kinetochore Okp1-Ame1 complex.EMBO reports · 2023Article
- Direct observation of coordinated assembly of individual native centromeric nucleosomes.The EMBO journal · 2023Article
- Review
- Cryo-EM structure of the complete inner kinetochore of the budding yeast point centromere.Science advances · 2023Article
- Structural mechanism of LIN28B nucleosome targeting by OCT4.Molecular cell · 2023Article
- Rio1 downregulates centromeric RNA levels to promote the timely assembly of structurally fit kinetochores.Nature communications · 2023Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
Accurate chromosome segregation relies on the specific centromeric nucleosome-kinetochore interface. In budding yeast, the centromere CBF3 complex guides the deposition of CENP-A, an H3 variant, to form the centromeric nucleosome in a DNA sequence-dependent manner. Here, we determine the structures of the centromeric nucleosome containing the native CEN3 DNA and the CBF3core bound to the canonical nucleosome containing an engineered CEN3 DNA. The centromeric nucleosome core structure contains 115 base pair DNA including a CCG motif. The CBF3core specifically recognizes the nucleosomal CCG motif through the Gal4 domain while allosterically altering the DNA conformation. Cryo-EM, modeling, and mutational studies reveal that the CBF3core forms dynamic interactions with core histones H2B and CENP-A in the CEN3 nucleosome. Our results provide insights into the structure of the budding yeast centromeric nucleosome and the mechanism of its assembly, which have implications for analogous processes of human centromeric nucleosome formation.
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