ArticleStem cell reports2021
Highly Efficient Genome Modification of Cultured Primordial Germ Cells with Lentiviral Vectors to Generate Transgenic Songbirds.
Article in Stem cell reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Short-term gonadal cultures are sufficient for germline transmission in a songbird.Stem cell reports · 2026Article
- Long-term rock dove (Columba livia) primordial germ cell culture: A tool towards avian conservation.The Journal of heredity · 2026Article
- Review
- Germ cell-directed exosomal delivery enables in vivo genetic modification in birds.Journal of nanobiotechnology · 2026Article
- Germline organoids develop in vitro from embryonic Taeniopygia guttata (zebra finch) cultures.Scientific reports · 2026Article
- Manipulating primordial germ cells from chicken and other bird species.The Journal of reproduction and development · 2026Review
- Are we truly helping those in need: A comprehensive assessment of semen technologies in wild bird species.Biodiversity and conservation · 2026Article
- Eye pigmentation-based in-ovo chicken sexing via precision breeding.Frontiers in bioengineering and biotechnology · 2026Article
- In Vitro Culture of Avian Primordial Germ Cells: Established Methods and Future Directions.Biology · 2025Review
- The barn owl in systems and behavioral neuroscience: Progress and promise.Current opinion in neurobiology · 2025Review
- Propagation of goose primordial germ cells in vitro relies on FGF and BMP signalling pathways.Communications biology · 2025Article
- Cryopreservation Strategies for Poultry Semen: A Comprehensive Review of Techniques and Applications.Veterinary sciences · 2025Review
- Toxic to the touch: The makings of lethal mantles in pitohui birds and poison dart frogs.Molecular ecology · 2024Article
- Review
- Induction of an immortalized songbird cell line allows for gene characterization and knockout by CRISPR-Cas9.Scientific reports · 2022Article
- A Model of Discovery: The Role of Imaging Established and Emerging Non-mammalian Models in Neuroscience.Frontiers in molecular neuroscience · 2022Review
- Efficient gene transfer into zebra finch germline-competent stem cells using an adenoviral vector system.Scientific reports · 2021Article
- Why zebra finches don't get hypercholesterolemia.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Single-Cell RNA Sequencing Revealed the Heterogeneity of Gonadal Primordial Germ Cells in Zebra Finch (Frontiers in cell and developmental biology · 2021Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability to genetically manipulate organisms has led to significant insights into functional genomics in many species. In birds, manipulation of the genome is hindered by the inaccessibility of the one-cell embryo. During embryonic development, avian primordial germ cells (PGCs) migrate through the bloodstream and reach the gonadal anlage, where they develop into mature germ cells. Here, we explored the use of PGCs to produce transgenic offspring in the zebra finch, which is a major animal model for sexual brain differentiation, vocal learning, and vocal communication. Zebra finch PGCs (zfPGCs) obtained from embryonic blood significantly proliferated when cultured in an optimized culture medium and conserved the expression of germ and stem cell markers. Transduction of cultured zfPGCs with lentiviral vectors was highly efficient, leading to strong expression of the enhanced green fluorescent protein. Transduced zfPGCs were injected into the host embryo and transgenic songbirds were successfully generated.
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