Evidence map›Paper›PMID 33740150›Full record

ArticleJournal of fluorescence2021

Effects of Viscosity and Refractive Index on the Emission and Diffusion Properties of Alexa Fluor 405 Using Fluorescence Correlation and Lifetime Spectroscopies.

Camila van Zanten, Dzmitry Melnikau, Alan G Ryder

Abstract read
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In one paragraph

Article in Journal of fluorescence, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Camila van ZantenNanoscale Biophotonics Laboratory, School of Chemistry, National University of Ireland ,Galway, University Road, Galway, H91 CF50, Ireland.ORCID http://orcid.org/0000-0002-5827-8561
Dzmitry MelnikauNanoscale Biophotonics Laboratory, School of Chemistry, National University of Ireland ,Galway, University Road, Galway, H91 CF50, Ireland.ORCID http://orcid.org/0000-0002-6588-8122
Alan G RyderNanoscale Biophotonics Laboratory, School of Chemistry, National University of Ireland ,Galway, University Road, Galway, H91 CF50, Ireland. alan.ryder@nuigalway.ie.ORCID http://orcid.org/0000-0002-3133-4340

Funding

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior BEX 1415-15-8Health Research Board EQ/2004/29Science Foundation Ireland 14/IA/2282
6 · The paper itself

Abstract

Fluorescence Correlation Spectroscopy (FCS) studies of the interaction of polymers or proteins in solution are strongly affected by the viscosity and refractive index of the medium, and the effects are likely to be more significant with the use of short wavelength excitation (e.g., 405 nm diode lasers). Failing to account for these issues can lead to incorrect measurement of average size, conformational changes, and dynamic behaviour of polymers and proteins. Steady-state, time-resolved, and FCS measurements of Alexa 405 in glycerol:water mixtures were performed to determine its suitability for FCS measurements with 405 nm excitation. The effects of the refractive index and viscosity on the diffusion coefficient and photophysical parameters (lifetime and relative quantum yield) of the fluorophore were determined. Alexa 405 lifetime decreased from 3.55 ns in water to 3.25 ns in a 50:50 glycerol:water mixture, while its diffusion coefficient dropped from 333 ± 16 to 44 ± 1 µm

Indexed as

Alexa Fluor 405Diffusion coefficientFluorescence correlation spectroscopyFluorescence lifetimeRefractive indexViscosity

Identifiers

PMID33740150

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.