Evidence map›Paper›PMID 33739251›Full record

ArticleThe Journal of general virology2021

Application of error-prone PCR to functionally probe the morbillivirus Haemagglutinin protein.

Giulia Gallo, Carina Conceicao, Christina Tsirigoti, Brian Willett, Stephen C Graham, Dalan Bailey

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of general virology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.3field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Giulia GalloThe Pirbright Institute, Guildford, Surrey, GU24 0NF, UK.
Carina ConceicaoThe Pirbright Institute, Guildford, Surrey, GU24 0NF, UK.
Christina TsirigotiThe Pirbright Institute, Guildford, Surrey, GU24 0NF, UK.
Brian WillettMRC University of Glasgow Centre for Virus Research, Glasgow, UK.
Stephen C GrahamDepartment of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK.
Dalan BaileyThe Pirbright Institute, Guildford, Surrey, GU24 0NF, UK.
The Pirbright Institute · GBMRC University of Glasgow Centre for Virus Research · GBUniversity of Cambridge · GB

Funding

Biotechnology and Biological Sciences Research Council BB/R019843/1Biotechnology and Biological Sciences Research Council BBS/E/I/00007030Biotechnology and Biological Sciences Research Council BBS/E/I/00007034Biotechnology and Biological Sciences Research Council BBS/E/I/00007039Medical Research Council MC_UU_12014/10Wellcome Trust
6 · The paper itself

Abstract

The enveloped morbilliviruses utilise conserved proteinaceous receptors to enter host cells: SLAMF1 or Nectin-4. Receptor binding is initiated by the viral attachment protein Haemagglutinin (H), with the viral Fusion protein (F) driving membrane fusion. Crystal structures of the prototypic morbillivirus measles virus H with either SLAMF1 or Nectin-4 are available and have served as the basis for improved understanding of this interaction. However, whether these interactions remain conserved throughout the morbillivirus genus requires further characterisation. Using a random mutagenesis approach, based on error-prone PCR, we targeted the putative receptor binding site for SLAMF1 interaction on peste des petits ruminants virus (PPRV) H, identifying mutations that inhibited virus-induced cell-cell fusion. These data, combined with structural modelling of the PPRV H and ovine SLAMF1 interaction, indicate this region is functionally conserved across all morbilliviruses. Error-prone PCR provides a powerful tool for functionally characterising functional domains within viral proteins.

Indexed as

AnimalsCell Adhesion MoleculesHemagglutinins, ViralHost Microbial InteractionsMembrane FusionPeste-des-petits-ruminants virusPolymerase Chain ReactionSheepSignaling Lymphocytic Activation Molecule Family Member 1Viral Fusion ProteinsCell Adhesion MoleculesHemagglutinins, ViralSignaling Lymphocytic Activation Molecule Family Member 1Viral Fusion Proteinsdirected evolutionepPCRmorbilliviruspeste des petits ruminants virusviral entryviral evolution

Identifiers

PMID33739251
PMCPMC8290269
OpenAlexW3136222140

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.