ArticleNano letters2021
High-Resolution Single-Molecule FRET via DNA eXchange (FRET X).
Article in Nano letters, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Impact of Molecular Crowding on Accessibility of Telomeric Overhangs Forming Multiple G-Quadruplexes.Biomacromolecules · 2025Article
- miRNA biomarkers: advancing early detection of acute myocardial infarction.Mikrochimica acta · 2025Review
- Impact of Molecular Crowding on Accessibility of Telomeric Overhangs Forming Multiple G-quadruplexes.bioRxiv : the preprint server for biology · 2025Article
- Decoding aptamer-protein binding kinetics for continuous biosensing using single-molecule techniques.Science advances · 2025Article
- Analyzing aptamer structure and interactions: in silico modelling and instrumental methods.Biophysical reviews · 2024Review
- Article
- Full-length single-molecule protein fingerprinting.Nature nanotechnology · 2024Article
- Topographic fingerprinting of single proteins and proteoforms.Nature nanotechnology · 2024Article
- Nanopore DNA sequencing technologies and their applications towards single-molecule proteomics.Nature chemistry · 2024Review
- The potential of fluorogenicity for single molecule FRET and DyeCycling.QRB discovery · 2024Review
- Single-molecule fluorescence methods for protein biomarker analysis.Analytical and bioanalytical chemistry · 2023Review
- Review of FRET biosensing and its application in biomolecular detection.American journal of translational research · 2023Review
- Protein Sequencing, One Molecule at a Time.Annual review of biophysics · 2022Review
- Can DyeCycling break the photobleaching limit in single-molecule FRET?Nano research · 2022Article
- Article
- Completing the canvas: advances and challenges for DNA-PAINT super-resolution imaging.Trends in biochemical sciences · 2021Review
- The emerging landscape of single-molecule protein sequencing technologies.Nature methods · 2021Review
- Single-Molecular Förster Resonance Energy Transfer Measurement on Structures and Interactions of Biomolecules.Micromachines · 2021Review
- FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices.eLife · 2021Review
- Quantitative Imaging With DNA-PAINT for Applications in Synaptic Neuroscience.Frontiers in synaptic neuroscience · 2021Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single-molecule FRET is a versatile tool to study nucleic acids and proteins at the nanometer scale. However, currently, only a couple of FRET pairs can be reliably measured on a single object, which makes it difficult to apply single-molecule FRET for structural analysis of biomolecules. Here, we present an approach that allows for the determination of multiple distances between FRET pairs in a single object. We use programmable, transient binding between short DNA strands to resolve the FRET efficiency of multiple fluorophore pairs. By allowing only a single FRET pair to be formed at a time, we can determine the pair distance with subnanometer precision. The distance between other pairs are determined by sequentially exchanging DNA strands. We name this multiplexing approach FRET X for FRET via DNA eXchange. Our FRET X technology will be a tool for the high-resolution analysis of biomolecules and nanostructures.
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