ArticleScientific reports2021
Transversal gene expression panel to evaluate intestinal health in broiler chickens in different challenging conditions.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 25 citations in OpenAlex.
- Article
- Optimized amino acid ratios in reduced crude protein diets sustain broiler performance, gut health, and resilience to necrotic enteritis.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2026Article
- Interactive effects of the feed-borne mycotoxin deoxynivalenol and a mixed-species Eimeria challenge on layer pullets during the transition to lay.Poultry science · 2026Article
- Synergistic effects of dietary RISCO-NUTRIFOUR probiotic on broiler performance, upregulation of intestinal immunoglobulin A and mucin-2 genes, enhancement of occludin expression, and downregulation of HSP70 under heat stress.Frontiers in veterinary science · 2026Article
- Tissue-specific transcriptomic adaptation in three strains of chickens during coinfections with parasites.Gut pathogens · 2025Article
- Effects of AddingAnimals : an open access journal from MDPI · 2025Article
- Modulation of gene expression in immune-related organs by in ovo stimulation with probiotics and prophybiotics in broiler chickens.Journal of applied genetics · 2025Article
- Effects of on-farm hatching versus hatchery hatching on growth performance, gut development, and intestinal health and function in broiler chickens.Poultry science · 2025Article
- Effect of β-Alanine Metabolite on Gut Integrity and Immunity in Commercial Broiler Chickens Infected withAnimals : an open access journal from MDPI · 2024Article
- Shifts in microbiota and gene expression of nutrient transporters, mucin and interleukins in the gut of fast-growing and slow-growing chickens infected by Salmonella Enteritidis.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Effect of diet supplemented with functional amino acids and polyphenols on gut health in broilers subjected to a corticosterone-induced stress.Scientific reports · 2024Article
- Composting: A biosecurity measure to maximize the benefit of broilers̕ litter.Journal of advanced veterinary and animal research · 2023Article
- Dietary Corn Silk (Animals : an open access journal from MDPI · 2023Article
- Effects ofMicroorganisms · 2023Article
- Evaluation of Predisposing Factors of Necrotic Enteritis in Experimentally Challenged Broiler Chickens.Animals : an open access journal from MDPI · 2022Article
- Article
- Structural Changes in Trabecular Bone, Cortical Bone and Hyaline Cartilage as Well as Disturbances in Bone Metabolism and Mineralization in an Animal Model of Secondary Osteoporosis inJournal of clinical medicine · 2021Article
- Article
- Impacts of increasing challenge with Eimeria maxima on the growth performance and gene expression of biomarkers associated with intestinal integrity and nutrient transporters.Veterinary research · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There is a high interest on gut health in poultry with special focus on consequences of the intestinal diseases, such as coccidiosis and C. perfringens-induced necrotic enteritis (NE). We developed a custom gene expression panel, which could provide a snapshot of gene expression variation under challenging conditions. Ileum gene expression studies were performed through high throughput reverse transcription quantitative real-time polymerase chain reaction. A deep review on the bibliography was done and genes related to intestinal health were selected for barrier function, immune response, oxidation, digestive hormones, nutrient transport, and metabolism. The panel was firstly tested by using a nutritional/Clostridium perfringens model of intestinal barrier failure (induced using commercial reused litter and wheat-based diets without exogenous supplementation of enzymes) and the consistency of results was evaluated by another experiment under a coccidiosis challenge (orally gavaged with a commercial coccidiosis vaccine, 90× vaccine dose). Growth traits and intestinal morphological analysis were performed to check the gut barrier failure occurrence. Results of ileum gene expression showed a higher expression in genes involved in barrier function and nutrient transport in chickens raised in healthy conditions, while genes involved in immune response presented higher expression in C.perfringens-challenged birds. On the other hand, the Eimeria challenge also altered the expression of genes related to barrier function and metabolism, and increased the expression of genes related to immune response and oxidative stress. The panel developed in the current study gives us an overview of genes and pathways involved in broiler response to pathogen challenge. It also allows us to deep into the study of differences in gene expression pattern and magnitude of responses under either a coccidial vaccine or a NE.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.