ArticlePloS one2021
Robust quantitative assessment of collagen fibers with picrosirius red stain and linearly polarized light as demonstrated on atherosclerotic plaque samples.
Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 26 citations in OpenAlex.
- Supervised Learning for Collagen Segmentation in Bright-Field Histology: A Comparative Evaluation of U-Net and MLP.Annals of biomedical engineering · 2026Article
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- EcoHIV Infection Promotes Atherosclerosis Progression in LDLR-Deficient Mice.Arteriosclerosis, thrombosis, and vascular biology · 2025Article
- The effect of the foreign body response on drug elution from subdermal delivery systems.Biomaterials · 2025Article
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- Quantitative analysis of fibrillar collagen organization in the immediate proximity of embedded fibroblasts in 3D collagen hydrogels.Journal of biosciences · 2024Article
- Characterization of the Temporomandibular Joint Disc Complex in the Yucatan Minipig.Tissue engineering. Part A · 2023Article
- The Remodeling of Dermal Collagen Fibrous Structures in Mice under Zero Gravity: The Role of Mast Cells.International journal of molecular sciences · 2023Article
- An inverse fitting strategy to determine the constrained mixture model parameters: application in patient-specific aorta.Frontiers in bioengineering and biotechnology · 2023Article
- Pegylated insulin-like growth factor-1 biotherapeutic delivery promotes rotator cuff regeneration in a rat model.Journal of biomedical materials research. Part A · 2022Article
- Human organ rejuvenation by VEGF-A: Lessons from the skin.Science advances · 2022Article
- 3DFrontiers in bioengineering and biotechnology · 2022Article
- Main Pathological Changes of Benign Ureteral Strictures.Frontiers in medicine · 2022Article
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Collagen is an important component in maintaining structural integrity and functionality of tissues and is modulated in various biological processes. Its visualization and possible quantification using histopathological stains can be important for understanding disease progression or therapeutic response. Visualization of collagen fiber with the histological stain picrosirius red (PSR) is enhanced with polarized light and quantitative analysis is possible using circular polarizers. However, linear polarizers are more commonly available and easier to optically align. The objective of the present study is to demonstrate a novel image acquisition technique and analysis method using linearly polarized light. The proposed imaging technique is based on image acquisition at multiple slide rotation angles, which are co-registered to form a composite image used for quantitative analysis by pixel intensity or pixel counting. The technique was demonstrated on multiple human coronary samples with varying histopathologies and developed specifically to analyze cap collagen in atherosclerotic plaque. Pixel counting image analysis was found to be reproducible across serial tissue sections and across different users and sufficiently sensitive to detect differences in cap structural integrity that are likely relevant to prediction of rupture risk. The benefit of slide rotation angle under linear polarization to acquire images represents a feasible and practical implementation for expanding the general utility of PSR for quantitative analysis.
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