ArticleACS chemical biology2021
Versatile Labeling and Detection of Endogenous Proteins Using Tag-Assisted Split Enzyme Complementation.
Article in ACS chemical biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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Who cites it
12 citing papers in PubMed.
- A high-affinity split-HaloTag for live-cell protein labeling.Nature communications · 2026Article
- Protein Engineering for Spatiotemporally Resolved Cellular Monitoring.Annual review of analytical chemistry (Palo Alto, Calif.) · 2025Review
- Experimental Approaches to Visualize Effector Protein Translocation During Host-Pathogen Interactions.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
- Principles and Design of Molecular Tools for Sensing and Perturbing Cell Surface Receptor Activity.Chemical reviews · 2025Review
- Unraveling the role of cAMP signaling inmSphere · 2024Article
- Single-molecule tracking (SMT): a window into live-cell transcription biochemistry.Biochemical Society transactions · 2023Article
- Rapid clonal identification of biallelic CRISPR/Cas9 knock-ins using SNEAK PEEC.Scientific reports · 2023Article
- Article
- Article
- Structural insights into the human PA28-20S proteasome enabled by efficient tagging and purification of endogenous proteins.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Vps13-like proteins provide phosphatidylethanolamine for GPI anchor synthesis in the ER.The Journal of cell biology · 2022Article
- Review
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Recent advances in genome engineering have expanded our capabilities to study proteins in their natural states. In particular, the ease and scalability of knocking-in small peptide tags has enabled high throughput tagging and analysis of endogenous proteins. To improve enrichment capacities and expand the functionality of knock-ins using short tags, we developed the tag-assisted split enzyme complementation (TASEC) approach, which uses two orthogonal small peptide tags and their cognate binders to conditionally drive complementation of a split enzyme upon labeled protein expression. Using this approach, we have engineered and optimized the tag-assisted split HaloTag complementation system (TA-splitHalo) and demonstrated its versatile applications in improving the efficiency of knock-in cell enrichment, detection of protein-protein interaction, and isolation of biallelic gene edited cells through multiplexing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.