ArticleInternational ophthalmology2021
Polysaccharides extracted from Cassia seeds protect against high glucose-induced retinal endothelial cell injury.
Article in International ophthalmology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed, 5 citations in OpenAlex.
- Elucidating the mechanism by which Aloe-emodin from Cassiae semen treats diabetic retinopathy via network pharmacology and experimental verification.Scientific reports · 2025Article
- Advances in health-promoting effects of natural polysaccharides: Regulation on Nrf2 antioxidant pathway.Frontiers in nutrition · 2023Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
introductionTo investigate the protect effect of polysaccharides extract from cassia seeds (CSPE) on human retinal endothelial cells (HRECs) in hyperglycemia environment.
methodsThe same amount of human retinal endothelial cells (HRECs), respectively, inoculated in vitro were divided into normal group (Con group), hyperglycemia group (H-Glu group), and different concentration of cassia polysaccharides extract (CSPE) combined with high glucose medium group (CSPE group). HRECs in Con group were cultured routinely. The cell in H-Glu group was treated with high glucose, in which the concentration of glucose in the medium was 30 mM. HRECs in CSPE group were treated with different concentrations of CSPE combined with high glucose. Enhanced cell counting kit-8(CCK8) assay was used to measure the HRECs cell survival rate in different groups. The generation of reactive oxygen species (ROS) in different group was measured by flow cytometry. The real-time quantitative PCR analysis was used for determining intracellular heme oxygenase-1 (HO-1) mRNA levels. Western Blot was applied to test the change of proteins, such as HO-1- and NF-E2-related factor 2 (Nrf2) protein.
resultsThe cell survival rate of the H-Glu group was significantly lower than that of the Con group (P < 0.05). When the concentration of CSPE was 100 mg/ml in CSPE group, the HRECs cell survival rate was significantly lower than that of the Con group (P < 0.05), and there was no significant difference with H-Glu group. When the concentration of CSPE in CSPE group was between 50 µg/ml and 1 × 10
conclusionsA certain concentration range of CSPE can increase the expression of the downstream protein HO-1 and negatively regulate the production of ROS by upregulating the expression of Nrf2, thus protecting human retinal endothelial cells from oxidative damage caused by high glucose.
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Registered trials
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