Evidence map›Paper›PMID 33732812›Full record

ArticleBio-protocol2021

Tracing Nitrogen Metabolism in Mouse Tissues with Gas Chromatography-Mass Spectrometry.

Rong Xu, Yekai Wang, Jianhai Du

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Rong XuDepartment of Ophthalmology and Visual Sciences, West Virginia University, Morgantown, USA.
Yekai WangDepartment of Ophthalmology and Visual Sciences, West Virginia University, Morgantown, USA.
Jianhai DuDepartment of Ophthalmology and Visual Sciences, West Virginia University, Morgantown, USA.
West Virginia University · US

Funding

Human RPE metabolism and metabolite transportR01EY026030 · NEI · UNIVERSITY OF WASHINGTON · PI CHAO, JENNIFER RAYMING, DU, JIANHAI · 2016 to 2020
$2.1M
Retinal Mitochondrial Metabolism in Alzheimer's DiseaseR01EY031324 · NEI · WEST VIRGINIA UNIVERSITY · PI DU, JIANHAI · 2021 to 2024
$2.0M
NEI NIH HHS R01 EY026030NEI NIH HHS R01 EY031324
6 · The paper itself

Abstract

Nitrogen-containing metabolites including ammonia, amino acids, and nucleotides, are essential for cell metabolism, growth, and neural transmission. Nitrogen metabolism is tightly coordinated with carbon metabolism in the breakdown and biosynthesis of amino acids and nucleotides. Both nuclear magnetic resonance spectroscopy and mass spectrometry including gas chromatography-mass spectrometry (GC MS) and liquid chromatography (LC MS) have been used to measure nitrogen metabolism. Here we describe a protocol to trace nitrogen metabolism in multiple mouse tissues using

Indexed as

15N tracingAmino acidsAmmoniaAmmonia metabolismGC MSMass spectrometryNitrogen metabolismStable isotope tracer

Identifiers

PMID33732812
PMCPMC7952953
OpenAlexW3132886946

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.