Evidence map›Paper›PMID 33731742›Full record

ArticleScientific reports2021

Comparison of three qPCR-based commercial tests for detection of periodontal pathogens.

Fridus Van der Weijden, Mirella Rijnen, Cees Valkenburg

Abstract readComparative Study
In one paragraph

Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Trial
  2. Trial
  3. Article
  4. Article
  5. Article
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Fridus Van der WeijdenClinic for Periodontology, Utrecht, The Netherlands. ga.vd.weijden@acta.nl.ORCID 0000-0002-5075-8384
Mirella RijnenClinic for Periodontology, Utrecht, The Netherlands.
Cees ValkenburgDepartment of Periodontology, Academic Centre for Dentistry Amsterdam (ACTA), A Joint Venture Between the Faculty of Dentistry, University of Amsterdam and Faculty of Dentistry, Vrije Universiteit Amsterdam, Gustav Mahlerlaan 3004, 1081 LA, Amsterdam, The Netherlands.ORCID 0000-0002-6656-6934

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In periodontal practice microbial results of periodontal test kits for identification of key pathogens are an aid in the treatment planning. Information on the performance of commercially available test kits is therefore essential for the clinician. In this retrospective analysis three commercially available qPCR kits for detection and quantification of selected periodontal bacterial species were compared, using 100 clinical samples from patients with untreated periodontitis. The analysis involved two separate comparisons in which kit A (LabOral Diagnostics, The Netherlands) was compared with kit B (Advanced Dental Diagnostics, The Netherlands), and with kit C (OralDent diagnostics, The Netherlands). Analytic procedures for detection and quantification of selected periodontal bacterial species were carried out according to the instructions of the laboratories. Kit A detected target species more often, and absolute numbers of bacterial cells were higher than with kit B. A high degree of similarity was found between the test outcomes by kit A and kit C. All three kits performed satisfactory but small and significant differences exist between kits.

Indexed as

PeriodontitisBacteriaDNA, BacterialHumansReagent Kits, DiagnosticReal-Time Polymerase Chain ReactionRetrospective StudiesDNA, BacterialReagent Kits, Diagnostic

Identifiers

PMID33731742
PMCPMC7969924

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.