ArticleScience advances2021
Multicolor three-photon fluorescence imaging with single-wavelength excitation deep in mouse brain.
Article in Science advances, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed.
- Lighting Up Biology: GFP-Inspired Fluorescent Probes for Sensing and Imaging.Journal of the American Chemical Society · 2026Article
- Giant anomalous exciton-multiphoton nonlinearities in layered hybrid perovskites.Light, science & applications · 2026Article
- Three-color high-definition whole-brain imaging: simultaneous analysis of multiple anatomical structures with colocalized cytoarchitectural information.Biomedical optics express · 2026Article
- Coumarin-Based Hybrids: From Linear Photophysical Properties to Multiphoton Excitation Behavior.ACS physical chemistry Au · 2026Article
- Multiplexed optoacoustic tracking and magnetic actuation of labeled blood cells in living mice.Science advances · 2026Article
- Multiplexed video hyperspectral microendoscopy reveals tumor-immune dynamics in vivo.Research square · 2026Article
- Simultaneous three-photon and optical coherence microscopy deep within an intact mouse brain.Npj imaging · 2026Article
- Three photon microscopy of mouse brain structure and function at 2 mm depth and beyond.bioRxiv : the preprint server for biology · 2026Article
- NIR Excitation in Atomically Precise Nanoclusters via Two-Photon and Three-Photon Absorption.Accounts of chemical research · 2026Article
- Multi-scale imaging and recording of in-vivo neural activity.BMB reports · 2026Review
- Longitudinal three-photon imaging for tracking amyloid plaques and vascular degeneration in a mouse model of Alzheimer's disease.Journal of biomedical optics · 2026Article
- Sub-50-femtosecond gain-managed amplified pulses enhance nonlinear ablation efficiency.Biomedical optics express · 2026Article
- Three-photon imaging of hippocampal neurogenesis through the intact mouse brain.Research square · 2025Article
- Molecular tools for decoding multicellular systems: from mechanisms to medicine.National science review · 2025Review
- Tissue type and tissue depth dependency of pulse broadening in three-photon microscopy: impact on third harmonic generation.Biomedical optics express · 2025Article
- Three-photon microscopy: an emerging technique for deep intravital brain imaging.Nature reviews. Neuroscience · 2025Review
- Multiplexed, image-based pooled screens in primary cells and tissues with PerturbView.Nature biotechnology · 2025Article
- Three-photon in vivo imaging of neurons and glia in the medial prefrontal cortex with sub-cellular resolution.Communications biology · 2025Article
- Deep-Penetrating and High-Resolution Continuous-Wave Nonlinear Microscopy Based on Homologous Dual-Emission Upconversion Adaptive Optics.Nano letters · 2025Article
- In vivo three-photon fluorescence imaging of mouse brain vasculature labeled by Evans blue excited at the NIR-III window.Biomedical optics express · 2025Article
2 more citing papers are in PubMed but not listed here.
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3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Multiphoton fluorescence microscopy is a powerful technique for deep-tissue observation of living cells. In particular, three-photon microscopy is highly beneficial for deep-tissue imaging because of the long excitation wavelength and the high nonlinear confinement in living tissues. Because of the large spectral separation of fluorophores of different color, multicolor three-photon imaging typically requires multiple excitation wavelengths. Here, we report a new three-photon excitation scheme: excitation to a higher-energy electronic excited state instead of the conventional excitation to the lowest-energy excited state, enabling multicolor three-photon fluorescence imaging with deep-tissue penetration in the living mouse brain using single-wavelength excitation. We further demonstrate that our excitation method results in ≥10-fold signal enhancement for some of the common red fluorescent molecules. The multicolor imaging capability and the possibility of enhanced three-photon excitation cross section will open new opportunities for life science applications of three-photon microscopy.
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