ArticleAngewandte Chemie (International ed. in English)2021
Reversible Ratiometric NADH Sensing Using Semiconducting Polymer Dots.
Article in Angewandte Chemie (International ed. in English), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 70 citations in OpenAlex.
- Fabrication of Co-Doped Covalent Organic Framework Nanosheets with Mild Interlayer Stress for Quantitative Detection of Alzheimer's Disease Biomarkers.Biosensors · 2026Article
- Hydrogen-bond-assisted meta-nitrogen-doped graphyne enables real-time electrocatalytic NADH tracking in single cells.Chemical science · 2026Article
- A Quinolinium-Functionalized Hemicyanine Dye for Ratiometric NAD(P)H Sensing in Live Cells, Kidney Tissues, and Drosophila melanogaster.Sensors and actuators. B, Chemical · 2025Article
- Methylquinolinium-enhanced near-infrared hemicyanine dye for ratiometric NAD(P)H sensing in live cellsJournal of materials chemistry. B · 2025Article
- Cyanine-Based Dual-Emission Sensor for Ratiometric NAD(P)H Imaging in Cells, Renal Tissues, and Entire Organisms.ACS applied bio materials · 2025Article
- High-Throughput Approaches to Engineer Fluorescent Nanosensors.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Semiconducting polymer dots for multifunctional integrated nanomedicine carriers.Materials today. Bio · 2024Review
- NAD(P)H Activated Fluorescent Probe for Rapid Intraoperative Pathological Diagnosis and Tumor Histological Grading.Chemical & biomedical imaging · 2023Article
- Illumination of Hydroxyl Radical in Kidney Injury and High-Throughput Screening of Natural Protectants Using a Fluorescent/Photoacoustic Probe.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Quantitative Galactose Colorimetric Competitive Assay Based on Galactose Dehydrogenase and Plasmonic Gold Nanostars.Biosensors · 2023Article
- π-Conjugated Polymer Nanoparticles from Design, Synthesis to Biomedical Applications: Sensing, Imaging, and Therapy.Microorganisms · 2023Review
- Ratiometric electrochemical OR gate assay for NSCLC-derived exosomes.Journal of nanobiotechnology · 2023Article
- Semiconducting Polymer Dots for Point-of-Care Biosensing and In Vivo Bioimaging: A Concise Review.Biosensors · 2023Review
- Recent Advances of NIR-II Emissive Semiconducting Polymer Dots for In Vivo Tumor Fluorescence Imaging and Theranostics.Biosensors · 2022Review
- Biomimetic semiconducting polymer dots for highly specific NIR-II fluorescence imaging of glioma.Materials today. Bio · 2022Article
- Construction of Enzyme-Responsive Micelles Based on Theranostic Zwitterionic Conjugated Bottlebrush Copolymers with Brush-on-Brush Architecture for Cell Imaging and Anticancer Drug Delivery.Molecules (Basel, Switzerland) · 2022Article
- Nanoparticulate Photoluminescent Probes for Bioimaging: Small Molecules and Polymers.International journal of molecular sciences · 2022Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
Reduced nicotinamide adenine dinucleotide (NADH) is a key coenzyme in living cells due to its role as an electron carrier in redox reactions, and its concentration is an important indicator of cell metabolic state. Abnormal NADH levels are associated with age-related metabolic diseases and neurodegenerative disorders, creating a demand for a simple, rapid analytical method for point-of-care NADH sensing. Here we develop a series of NADH-sensitive semiconducting polymer dots (Pdots) as nanoprobes for NADH measurement, and test their performance in vitro and in vivo. NADH sensing is based on electron transfer from semiconducting polymer chains in the Pdot to NADH upon UV excitation, quenching Pdot fluorescence emission. In polyfluorene-based Pdots, this mechanism resulted in an on-off NADH sensor; in DPA-CNPPV Pdots, UV excitation resulted in NADH-sensitive emission at two wavelengths, enabling ratiometric detection. Ratiometric NADH detection using DPA-CNPPV Pdots exhibits high sensitivity (3.1 μM limit of detection), excellent selectivity versus other analytes, reversibility, and a fast response (less than 5 s). We demonstrate applications of the ratiometric NADH-sensing Pdots including smartphone-based NADH imaging for point-of-care use.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.