ArticleInternational journal of epidemiology2021
Assessing the role of genome-wide DNA methylation between smoking and risk of lung cancer using repeated measurements: the HUNT study.
Article in International journal of epidemiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 2 of them syntheses that pooled it.
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Who cites it
20 citing papers in PubMed, 2 syntheses or guidelines pooled it, 32 citations in OpenAlex.
- Pooled it
- Meta-analysis of whole-genome gene expression datasets assessing the effects of IDH1 and IDH2 mutations in isogenic disease models.Scientific reports · 2022Pooled it
- Blood-based DNA methylation marker model for short-term and long-term lung cancer risk prediction.BMC medicine · 2026Article
- Association between pace of aging estimated from blood DNA methylation and all-cause mortality: the HUNT study.Clinical epigenetics · 2026Article
- Genome-wide profiling of salivary promoter-region DNA methylation in periodontitis: the Tromsø Study.BMC medical genomics · 2026Article
- CoxMDS: multiple data splitting for high-dimensional mediation analysis with survival outcomes in epigenome-wide studies.Briefings in bioinformatics · 2026Article
- Epigenetic Alterations Induced by Smoking and Their Intersection with Artificial Intelligence: A Narrative Review.International journal of environmental research and public health · 2025Review
- DNA methylation profiles of long-term cannabis users in midlife: a comprehensive evaluation of published cannabis-associated methylation markers in a representative cohort.Molecular psychiatry · 2025Article
- Unidirectional and bidirectional causation between smoking and blood DNA methylation: evidence from twin-based Mendelian randomisation.European journal of epidemiology · 2025Article
- Unidirectional and Bidirectional Causation between Smoking and Blood DNA Methylation: Evidence from Twin-based Mendelian Randomisation.medRxiv : the preprint server for health sciences · 2024Article
- Epigenome-wide association study of total nicotine equivalents in multiethnic current smokers from three prospective cohorts.American journal of human genetics · 2024Article
- Blood-based DNA methylation markers for lung cancer prediction.BMJ oncology · 2024Article
- Increased expression of individual genes in whole blood is associated with late-stage lung cancer at and close to diagnosis.Scientific reports · 2023Article
- Smoking, blood DNA methylation sites and lung cancer risk.Environmental pollution (Barking, Essex : 1987) · 2023Article
- Genome-wide DNA methylation association study of recent and cumulative marijuana use in middle aged adults.Molecular psychiatry · 2023Article
- Refining epigenetic prediction of chronological and biological age.Genome medicine · 2023Article
- DNA-Methylation Signatures of Tobacco Smoking in a High Cardiovascular Risk Population: Modulation by the Mediterranean Diet.International journal of environmental research and public health · 2023Article
- Characterisation of ethnic differences in DNA methylation between UK-resident South Asians and Europeans.Clinical epigenetics · 2022Article
- Applying Mendelian randomization to appraise causality in relationships between nutrition and cancer.Cancer causes & control : CCC · 2022Article
- Smoking, DNA Methylation, and Breast Cancer: A Mendelian Randomization Study.Frontiers in oncology · 2021Article
Corrections and comments
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Authors and funding
21 authors at 8 institutions in 5 countries.
Funding
Abstract
backgroundIt is unclear if smoking-related DNA methylation represents a causal pathway between smoking and risk of lung cancer. We sought to identify novel smoking-related DNA methylation sites in blood, with repeated measurements, and to appraise the putative role of DNA methylation in the pathway between smoking and lung cancer development.
methodsWe derived a nested case-control study from the Trøndelag Health Study (HUNT), including 140 incident patients who developed lung cancer during 2009-13 and 140 controls. We profiled 850 K DNA methylation sites (Illumina Infinium EPIC array) in DNA extracted from blood that was collected in HUNT2 (1995-97) and HUNT3 (2006-08) for the same individuals. Epigenome-wide association studies (EWAS) were performed for a detailed smoking phenotype and for lung cancer. Two-step Mendelian randomization (MR) analyses were performed to assess the potential causal effect of smoking on DNA methylation as well as of DNA methylation (13 sites as putative mediators) on risk of lung cancer.
resultsThe EWAS for smoking in HUNT2 identified associations at 76 DNA methylation sites (P < 5 × 10-8), including 16 novel sites. Smoking was associated with DNA hypomethylation in a dose-response relationship among 83% of the 76 sites, which was confirmed by analyses using repeated measurements from blood that was collected at 11 years apart for the same individuals. Two-step MR analyses showed evidence for a causal effect of smoking on DNA methylation but no evidence for a causal link between DNA methylation and the risk of lung cancer.
conclusionsDNA methylation modifications in blood did not seem to represent a causal pathway linking smoking and the lung cancer risk.
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