ArticleMemorias do Instituto Oswaldo Cruz2021
Validation of ELISA with recombinant antigens in serological diagnosis of canine Leishmania infantum infection.
Article in Memorias do Instituto Oswaldo Cruz, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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Who cites it
8 citing papers in PubMed, 14 citations in OpenAlex.
- Neglected parasitic diseases from a one-health perspective: American trypanosomiasis and leishmaniasis in dogs and humans in the Bolivian Chaco.Parasites & vectors · 2025Article
- Performance of Extracellular Vesicles FromJournal of parasitology research · 2025Article
- Baculovirus-Assisted Production ofPathogens (Basel, Switzerland) · 2024Article
- Rapid and reliable detection of Leishmania antibodies in canine serum with double-antigen sandwich homogeneous chemical luminescence.Parasites & vectors · 2024Article
- Article
- Recombinant protein KR95 as an alternative for serological diagnosis of human visceral leishmaniasis in the Americas.PloS one · 2023Article
- Use of Antigen Combinations to Address ComplexMicroorganisms · 2022Article
- Canine Leishmaniasis: Update on Epidemiology, Diagnosis, Treatment, and Prevention.Veterinary sciences · 2022Review
Corrections and comments
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Authors and funding
14 authors at 7 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDogs are the main peridomiciliary reservoir of Leishmania infantum thus the correct diagnosis of infection is essential for the control of the transmission and treatment as well. However, the diagnosis is based on serological assays that are not fully effective.
objectiveWe aimed to establish an effective serological assay for the diagnosis of L. infantum infected dogs using Leishmania-derived recombinant antigens.
methodsLeishmania derived rK39-, rK28-, rKR95-based enzyme-linked immunosorbent assay (ELISA) was standardized using symptomatic and asymptomatic L. infantum-infected dogs. Then 2,530 samples from inquiry in endemic areas for VL were evaluated and the results compared with recommended assays by the Brazilian Ministry of Health (MH algorithm). Further samples from a cohort of 30 dogs were searched.
findingsFor rK39-, rK28- and rKR95-ELISA the sensitivity was around 97% and specificity 100%. The positivity of these three ELISA in the inquiry samples was 27-28%, around 10% higher than the assays currently in use. When cohort samples were searched, we observed likely false-negative results (> 65%) with supposedly negative samples that turned positive six months later with the assays in use (MH algorithm). MAIN
conclusionsFor the diagnosis of L. infantum-infected dogs, rK39-based ELISA showed better diagnostic performance than other assays in use in Brazil and worldwide.
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