ArticleSoft matter2021
Cryo-EM is a powerful tool, but helical applications can have pitfalls.
Article in Soft matter, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Unravelling emergence of chirality in click-chemistry polymers down to the single-chain level.Nature communications · 2025Article
- Purification and Cryo-Electron Microscopy Analysis of Bacterial Appendages.Bio-protocol · 2024Article
- DeepTracer-ID: De novo protein identification from cryo-EM maps.Biophysical journal · 2022Article
- Helical Indexing in Real Space.Scientific reports · 2022Article
- Atomic structure of Lanreotide nanotubes revealed by cryo-EM.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Python-based Helix Indexer: A graphical user interface program for finding symmetry of helical assembly through Fourier-Bessel indexing of electron microscopic data.Protein science : a publication of the Protein Society · 2022Article
- Cryo-electron tomography related radiation-damage parameters for individual-molecule 3D structure determination.Frontiers in chemistry · 2022Review
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
In structural biology, cryo-electron microscopy (cryo-EM) has emerged as the main technique for determining the atomic structures of macromolecular complexes. This has largely been due to the introduction of direct electron detectors, which have allowed for routinely reaching a near-atomic resolution when imaging such complexes. In chemistry and materials science, the applications of cryo-EM have been much more limited. A recent paper (Z. Li et al., Chemically Controlled Helical Polymorphism In Protein Tubes By Selective Modulation Of Supramolecular Interactions, J. Am. Chem. Soc. 2019, 141, 19448-19457) has used low resolution cryo-EM to analyze polymorphic helical tubes formed by a tetrameric protein, and has made detailed models for the interfaces between the tetramers in these assemblies. Due to intrinsic ambiguities in determining the correct helical symmetry, we show that many of the models are likely to be wrong. This note highlights both the enormous potential for using cryo-EM, and also the pitfalls possible for helical assemblies when a near-atomic level of resolution is not reached.
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Registered trials
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