Evidence map›Paper›PMID 33727224›Full record

ArticleRNA (New York, N.Y.)2021

An improved in vivo tethering assay with single molecule FISH reveals that a nematode Nanos enhances reporter expression and mRNA stability.

Jonathan Doenier, Tina R Lynch, Judith Kimble, Scott T Aoki

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in RNA (New York, N.Y.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 63% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Jonathan Doenier *Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
Tina R Lynch *Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
Judith KimbleDepartment of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
Scott T AokiDepartment of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
University of Wisconsin–Madison · US

Funding

Molecular regulation of germline developmentR01GM134119 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI KIMBLE, JUDITH · 2019 to 2022
$2.2M
The mechanics and dynamics of RNP granulesK99HD081208 · NICHD · UNIVERSITY OF WISCONSIN-MADISON · PI AOKI, SCOTT T · 2015 to 2016
$202k
Howard Hughes Medical InstituteNICHD NIH HHS K99 HD081208NIGMS NIH HHS R01 GM134119
6 · The paper itself

Abstract

Robust methods are critical for testing the in vivo regulatory mechanism of RNA binding proteins. Here we report improvement of a protein-mRNA tethering assay to probe the function of an RNA binding protein in its natural context within the

Indexed as

RNA StabilityAnimalsCaenorhabditis elegansCaenorhabditis elegans ProteinsGene Expression RegulationGerm CellsIn Situ Hybridization, FluorescenceRNA, MessengerSingle Molecule ImagingCaenorhabditis elegans ProteinsNos-3 protein, C elegansRNA, MessengerC. elegansgermlineNanosNOS-3RNA binding protein

Identifiers

PMID33727224
PMCPMC8127996
OpenAlexW3137242376

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.