ArticleBiotechnology for biofuels2021
Overproduction of docosahexaenoic acid in Schizochytrium sp. through genetic engineering of oxidative stress defense pathways.
Article in Biotechnology for biofuels, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 36 citations in OpenAlex.
- Enhancing ε-poly-L-lysine production in Streptomyces albulus through tea polyphenols addition.Bioprocess and biosystems engineering · 2026Article
- Algal metabolites as emerging anti-virulence therapeutics against Pseudomonas aeruginosa.Archives of microbiology · 2025Review
- A Review on Marine Microbial Docosahexaenoic Acid Production Through Circular Economy, Fermentation Engineering, and Antioxidant Technology.Marine drugs · 2025Review
- Metabolic regulation strategies for enhancing microbial docosahexaenoic acid production by Schizochytrium sp.World journal of microbiology & biotechnology · 2025Review
- Engineering Fatty Acid Biosynthesis in Microalgae: Recent Progress and Perspectives.Marine drugs · 2024Review
- Development of Schizochytrium sp. strain HS01 with high-DHA and low-saturated fatty acids production by multi-pronged adaptive evolution.Biotechnology letters · 2023Article
- Zinc Finger Protein LipR Represses Docosahexaenoic Acid and Lipid Biosynthesis inApplied and environmental microbiology · 2022Article
- Propanol and 1, 3-propanediol enhance fatty acid accumulation synergistically inFrontiers in microbiology · 2022Article
- Deciphering and engineering the polyunsaturated fatty acid synthase pathway from eukaryotic microorganisms.Frontiers in bioengineering and biotechnology · 2022Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundOxidation and peroxidation of lipids in microorganisms result in increased levels of intracellular reactive oxygen species (ROS) and reactive aldehydes, and consequent reduction of cell growth and lipid accumulation.
resultsTo reduce oxygen-mediated cell damage and increase lipid and docosahexaenoic acid (DHA) production in Schizochytrium sp., we strengthened the oxidative stress defense pathways. Overexpression of the enzymes thioredoxin reductase (TRXR), aldehyde dehydrogenase (ALDH), glutathione peroxidase (GPO), and glucose-6-phosphate dehydrogenase (ZWF) strongly promoted cell growth, lipid yield, and DHA production. Coexpression of ZWF, ALDH, GPO, and TRXR enhanced ROS-scavenging ability. Highest values of dry cell weight, lipid yield, and DHA production (50.5 g/L, 33.1 g/L, and 13.3 g/L, respectively) were attained in engineered strain OaldH-gpo-trxR by shake flask fed-batch culture; these were increases of 18.5%, 80.9%, and 114.5% relative to WT values.
conclusionsOur findings demonstrate that engineering of oxidative stress defense pathways is an effective strategy for promoting cell robustness, lipid yield, and DHA production in Schizochytrium.
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