Evidence map›Paper›PMID 33726789›Full record

ArticleMolecular brain2021

The C. elegans homolog of human panic-disorder risk gene TMEM132D orchestrates neuronal morphogenesis through the WAVE-regulatory complex.

Xin Wang, Wei Jiang, Shuo Luo, Xiaoyu Yang, Changnan Wang, Bingying Wang, Yongjun Dang, Yin Shen, Dengke K Ma

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular brain, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
0.8field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 10 citations in OpenAlex.

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  9. Finding New Ways How to Control BACE1.The Journal of membrane biology · 2022
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 2 countries.

Xin Wang *Cardiovascular Research Institute and Department of Physiology, University of California San Francisco, San Francisco, CA, 94158, USA.
Wei Jiang *Key Laboratory of Metabolism and Molecular Medicine, the Ministry of Education, Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Shanghai Medical College of Fudan University, Fudan University, Shanghai, 200032, China.
Shuo LuoCardiovascular Research Institute and Department of Physiology, University of California San Francisco, San Francisco, CA, 94158, USA.
Xiaoyu YangInstitute for Human Genetics, Department of Neurology, University of California San Francisco, San Francisco, CA, 94158, USA.
Changnan WangCardiovascular Research Institute and Department of Physiology, University of California San Francisco, San Francisco, CA, 94158, USA.
Bingying WangCardiovascular Research Institute and Department of Physiology, University of California San Francisco, San Francisco, CA, 94158, USA.
Yongjun DangKey Laboratory of Metabolism and Molecular Medicine, the Ministry of Education, Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Shanghai Medical College of Fudan University, Fudan University, Shanghai, 200032, China.
Yin ShenInstitute for Human Genetics, Department of Neurology, University of California San Francisco, San Francisco, CA, 94158, USA.
Dengke K MaCardiovascular Research Institute and Department of Physiology, University of California San Francisco, San Francisco, CA, 94158, USA. Dengke.Ma@ucsf.edu.ORCID 0000-0002-5619-7485
University of California, San Francisco · USShanghai Medical College of Fudan University · CN

Funding

Dissecting a Novel Genetic Pathway for Fatty Acid Desaturation and Temperature AdaptationR01GM117461 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI MA, DENGKE · 2016 to 2020
$1.6M
NIGMS NIH HHS R01 GM117461
6 · The paper itself

Abstract

TMEM132D is a human gene identified with multiple risk alleles for panic disorders, anxiety and major depressive disorders. Defining a conserved family of transmembrane proteins, TMEM132D and its homologs are still of unknown molecular functions. By generating loss-of-function mutants of the sole TMEM132 ortholog in C. elegans, we identify abnormal morphologic phenotypes in the dopaminergic PDE neurons. Using a yeast two-hybrid screen, we find that NAP1 directly interacts with the cytoplasmic domain of human TMEM132D, and mutations in C. elegans tmem-132 that disrupt interaction with NAP1 cause similar morphologic defects in the PDE neurons. NAP1 is a component of the WAVE regulatory complex (WRC) that controls F-actin cytoskeletal dynamics. Decreasing activity of WRC rescues the PDE defects in tmem-132 mutants, whereas gain-of-function of TMEM132D in mammalian cells inhibits WRC, leading to decreased abundance of select WRC components, impaired actin nucleation and cell motility. We propose that metazoan TMEM132 family proteins play evolutionarily conserved roles in regulating NAP1 protein homologs to restrict inappropriate WRC activity, cytoskeletal and morphologic changes in the cell.

Indexed as

ActinsAnimalsBiological EvolutionCaenorhabditis elegansCaenorhabditis elegans ProteinsCell ShapeConserved SequenceCytoskeletonDopaminergic NeuronsGain of Function MutationGenes, ReporterHEK293 CellsHumansLoss of Function MutationMembrane ProteinsMorphogenesisActinsCaenorhabditis elegans ProteinsMembrane ProteinsMultiprotein ComplexesRecombinant Fusion ProteinsTMEM132D protein, humanActinC. elegansPanic disorderTMEM132DWAVE regulatory complex

Identifiers

PMID33726789
PMCPMC7962252
OpenAlexW3136084215

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.