ArticleBMC plant biology2021
Knockout of the entire family of AITR genes in Arabidopsis leads to enhanced drought and salinity tolerance without fitness costs.
Article in BMC plant biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers, 1 of them a synthesis that pooled it.
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Who cites it
43 citing papers in PubMed, 1 synthesis or guideline pooled it, 97 citations in OpenAlex.
- Uncovering core regulators of multi-abiotic stress adaptation in Arabidopsis thaliana through integrative meta-analysis and machine learning with RT-qPCR validation.Scientific reports · 2026Pooled it
- Research progress in gene editing of Brassicaceae crops.Biodesign research · 2026Review
- Gene network analysis predicts the primary regulators of ABA-dependent transcriptional activation and repression in Populus roots.PLoS genetics · 2026Article
- Crops exposed to extreme conditions: perspectives of gene editing to improve stress tolerance.Plant cell reports · 2026Review
- Genome-wide identification and functional characterization of ABA and salt stress responsive AITRs transcriptional repressors genes in sweet potato (Ipomoea batatas).BMC plant biology · 2026Article
- Overexpression of PtrPYL11 enhance drought tolerance in Populus: evidence from genome-wide identification and comparative transcriptome profiling.Plant cell reports · 2026Article
- AtAUGs Suppress the Expression of PP2C Genes to Redundantly Regulate ABA Responses in Arabidopsis.Plants (Basel, Switzerland) · 2026Article
- Harnessing Antioxidants for Abiotic Stress Management: Mechanistic Insights and Prospects for Sustainable Agriculture.Antioxidants (Basel, Switzerland) · 2026Review
- Plant Adaptation to Salinity: Physiological Pathways and Prospects for Crop Improvement-A Review.Genes · 2026Review
- Next-generation CRISPR systems and advanced delivery paradigms for precision crop improvement: a comparative case study in sugar beet (Frontiers in plant science · 2026Review
- Functional and Mechanistic Insights into Plant VQ Proteins in Abiotic and Biotic Stress Responses.Plants (Basel, Switzerland) · 2025Review
- Integrating Drought Stress Signaling and Smart Breeding for Climate-Resilient Crops: Regulatory Mechanisms and Genetic Strategies.Plants (Basel, Switzerland) · 2025Review
- Unlocking gene regulatory networks for crop resilience and sustainable agriculture.Nature biotechnology · 2025Review
- Article
- Stress Increases Ecological Risk of Glufosinate-Resistant Transgene Located on Alien Chromosomes in Hybrids Between TransgenicPlants (Basel, Switzerland) · 2025Article
- Emerging applications of gene editing technologies for the development of climate-resilient crops.Frontiers in genome editing · 2025Review
- Applications of CRISPR/Cas tools in improving stress tolerance inFrontiers in plant science · 2025Review
- Article
- CRISPR/Cas9 mediated genome editing for crop improvement against Abiotic stresses: current trends and prospects.Functional & integrative genomics · 2024Review
- A review on strategies for crop improvement against drought stress through molecular insights.3 Biotech · 2024Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
backgorundEnvironmental stresses including abiotic stresses and biotic stresses limit yield of plants. Stress-tolerant breeding is an efficient way to improve plant yield under stress conditions. Genome editing by CRISPR/Cas9 can be used in molecular breeding to improve agronomic traits in crops, but in most cases, with fitness costs. The plant hormone ABA regulates plant responses to abiotic stresses via signaling transduction. We previously identified AITRs as a family of novel transcription factors that play a role in regulating plant responses to ABA and abiotic stresses. We found that abiotic stress tolerance was increased in the single, double and triple aitr mutants. However, it is unclear if the increased abiotic stress tolerance in the mutants may have fitness costs.
resultsWe report here the characterization of AITRs as suitable candidate genes for CRISPR/Cas9 editing to improve plant stress tolerance. By using CRISPR/Cas9 to target AITR3 and AITR4 simultaneously in the aitr256 triple and aitr1256 quadruple mutants respectively, we generated Cas9-free aitr23456 quintuple and aitr123456 sextuple mutants. We found that reduced sensitivities to ABA and enhanced tolerance to drought and salt were observed in these mutants. Most importantly, plant growth and development was not affected even in the aitr123456 sextuple mutants, in whom the entire AITR family genes have been knocked out, and the aitr123456 sextuple mutants also showed a wild type response to the pathogen infection.
conclusionsOur results suggest that knockout of the AITR family genes in Arabidopsis enhanced abiotic stress tolerance without fitness costs. Considering that knock-out a few AITRs will lead to enhanced abiotic stress tolerance, that AITRs are widely distributed in angiosperms with multiple encoding genes, AITRs may be targeted for molecular breeding to improve abiotic stress tolerance in plants including crops.
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